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dc.contributor.authorModrell, MSen
dc.contributor.authorLyne, Men
dc.contributor.authorCarr, ARen
dc.contributor.authorZakon, HHen
dc.contributor.authorBuckley, Den
dc.contributor.authorCampbell, ASen
dc.contributor.authorDavis, MCen
dc.contributor.authorMicklem, Gosen
dc.contributor.authorBaker, Clareen
dc.date.accessioned2017-06-15T10:09:50Z
dc.date.available2017-06-15T10:09:50Z
dc.date.issued2017-03-27en
dc.identifier.issn2050-084X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/264780
dc.description.abstractThe anamniote lateral line system, comprising mechanosensory neuromasts and electrosensory ampullary organs, is a useful model for investigating the developmental and evolutionary diversification of different organs and cell types. Zebrafish neuromast development is increasingly well understood, but neither zebrafish nor $\textit{Xenopus}$ is electroreceptive and our molecular understanding of ampullary organ development is rudimentary. We have used RNA-seq to generate a lateral line-enriched gene-set from late-larval paddlefish ($\textit{Polyodon spathula}$). Validation of a subset reveals expression in developing ampullary organs of transcription factor genes critical for hair cell development, and genes essential for glutamate release at hair cell ribbon synapses, suggesting close developmental, physiological and evolutionary links between non-teleost electroreceptors and hair cells. We identify an ampullary organ-specific proneural transcription factor, and candidates for the voltage-sensing L-type Ca$_v$ channel and rectifying K$_v$ channel predicted from skate (cartilaginous fish) ampullary organ electrophysiology. Overall, our results illuminate ampullary organ development, physiology and evolution.
dc.description.sponsorshipBiotechnology and Biological Sciences Research Council (BB/F00818X/1), Leverhulme Trust (RPG-383), Fisheries Society of the British Isles (Research Grant), National Science Foundation (IOS 1557857, IOS 1144965)
dc.languageengen
dc.language.isoenen
dc.publishereLife Sciences Publications Ltd
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAtoh1en
dc.subjectCav1.3en
dc.subjectCavβ2en
dc.subjectKv1.5en
dc.subjectKvβ3en
dc.subjectNeurod4en
dc.subjectPolyodon spathula (Mississippi paddlefish)en
dc.subjectPou4f3en
dc.subjectVglut3en
dc.subjectampullary organsen
dc.subjectbeta-parvalbuminsen
dc.subjectdevelopmental biologyen
dc.subjectelectroreceptorsen
dc.subjecthair cellsen
dc.subjecthhen
dc.subjectneuromastsen
dc.subjectneuroscienceen
dc.subjectoncomodulinen
dc.subjectotoferlinen
dc.subjectstem cellsen
dc.subjectsynaptic ribbonsen
dc.subjectvoltage-gated ion channelsen
dc.titleInsights into electrosensory organ development, physiology and evolution from a lateral line-enriched transcriptomeen
dc.typeArticle
prism.numbere24197en
prism.publicationDate2017en
prism.publicationNameeLifeen
prism.volume6en
dc.identifier.doi10.17863/CAM.10473
dcterms.dateAccepted2017-03-23en
rioxxterms.versionofrecord10.7554/eLife.24197en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-03-27en
dc.contributor.orcidMicklem, Gos [0000-0002-6883-6168]
dc.contributor.orcidBaker, Clare [0000-0002-4434-3107]
dc.identifier.eissn2050-084X
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/F00818X/1)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International