dc.contributor.author Abkenar, M en dc.contributor.author Gray, Thomas en dc.contributor.author Zaccone, Alessio en dc.date.accessioned 2017-07-03T14:28:20Z dc.date.available 2017-07-03T14:28:20Z dc.date.issued 2017-04-28 en dc.identifier.issn 2470-0045 dc.identifier.uri https://www.repository.cam.ac.uk/handle/1810/265132 dc.description.abstract Theories that are used to extract energy-landscape information from single-molecule pulling experiments in biophysics are all invariably based on Kramers' theory of the thermally activated escape rate from a potential well. As is well known, this theory recovers the Arrhenius dependence of the rate on the barrier energy and crucially relies on the assumption that the barrier energy is much larger than $k_\text{B}T$ (limit of comparatively low thermal fluctuations). As was shown already in Dudko $\textit{et al.}$ [Phys. Rev. Lett. 96, 108101 (2006)], this approach leads to the unphysical prediction of dissociation time increasing with decreasing binding energy when the latter is lowered to values comparable to $k_\text{B}T$ (limit of large thermal fluctuations). We propose a theoretical framework (fully supported by numerical simulations) which amends Kramers' theory in this limit and use it to extract the dissociation rate from single-molecule experiments where now predictions are physically meaningful and in agreement with simulations over the whole range of applied forces (binding energies). These results are expected to be relevant for a large number of experimental settings in single-molecule biophysics. dc.description.sponsorship EPSRC (Ph.D. studentship) dc.language eng en dc.language.iso en en dc.publisher American Physical Society dc.title Dissociation rates from single-molecule pulling experiments under large thermal fluctuations or large applied force en dc.type Article prism.issueIdentifier 4 en prism.number 042413 en prism.publicationDate 2017 en prism.publicationName Physical Review E - Statistical, Nonlinear, and Soft Matter Physics en prism.volume 95 en dc.identifier.doi 10.17863/CAM.11161 dcterms.dateAccepted 2017-04-03 en rioxxterms.versionofrecord 10.1103/PhysRevE.95.042413 en rioxxterms.version AM en rioxxterms.licenseref.uri http://www.rioxx.net/licenses/all-rights-reserved en rioxxterms.licenseref.startdate 2017-04-28 en dc.contributor.orcid Gray, Thomas [0000-0003-1975-1042] dc.identifier.eissn 2470-0053 rioxxterms.type Journal Article/Review en pubs.funder-project-id EPSRC (1778174)
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