Show simple item record

dc.contributor.authorBell, Samuelen
dc.contributor.authorTerentjev, Eugeneen
dc.date.accessioned2017-07-04T10:49:16Z
dc.date.available2017-07-04T10:49:16Z
dc.date.issued2017-06-06en
dc.identifier.issn0006-3495
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/265145
dc.description.abstractSensors are the first element of the pathways that control the response of cells to their environment. Protein complexes that produce or enable a chemical signal in response to a mechanical stimulus are called ‘‘mechanosensors’’. In this work, we develop a theoretical model describing the physical mechanism of a reversible single-molecule stiffness sensor. Although this has the potential for general application, here we apply the model to focal adhesion kinase, which initiates the chemical signal in its active phosphorylated conformation, but can spontaneously return to its closed folded conformation. We find how the rates of conformation changes depend on the substrate stiffness and the pulling force applied from the cell cytoskeleton. We find the sensor is homeostatic, spontaneously self-adjusting to reach a state where its range of maximum sensitivity matches the substrate stiffness. The results compare well with the phenotype observations of cells on different substrates.
dc.description.sponsorshipThis work has been funded by Engineering and Physical Sciences Research Council (EPSRC) grant No. EP/M508007/1.
dc.language.isoenen
dc.publisherElsevier (Cell Press)
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.titleFocal Adhesion Kinase: The Reversible Molecular Mechanosensoren
dc.typeArticle
prism.endingPage2450
prism.issueIdentifier11en
prism.publicationDate2017en
prism.publicationNameBiophysical Journalen
prism.startingPage2439
prism.volume112en
dc.identifier.doi10.17863/CAM.10546
dcterms.dateAccepted2017-04-28en
rioxxterms.versionofrecord10.1016/j.bpj.2017.04.048en
rioxxterms.versionAMen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
rioxxterms.licenseref.startdate2017-06-06en
dc.contributor.orcidBell, Samuel [0000-0001-5475-4025]
dc.contributor.orcidTerentjev, Eugene [0000-0003-3517-6578]
dc.identifier.eissn1542-0086
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEPSRC (EP/F032773/1)
pubs.funder-project-idEPSRC (EP/M508007/1)
cam.issuedOnline2017-06-11en
datacite.issupplementedby.doi10.1016/j.bpj.2017.04.048en
cam.orpheus.successThu Jan 30 12:53:30 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International