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dc.contributor.authorErard, Nicolasen
dc.contributor.authorKnott, SRVen
dc.contributor.authorHannon, Gregoryen
dc.date.accessioned2017-07-12T10:44:12Z
dc.date.available2017-07-12T10:44:12Z
dc.date.issued2017-07-20en
dc.identifier.issn1097-2765
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/265269
dc.description.abstractWe have combined a machine-learning approach with other strategies to optimize knockout efficiency with the CRISPR/Cas9 system. In addition, we have developed a multiplexed sgRNA expression strategy that promotes the functional ablation of single genes and allows for combinatorial targeting. These strategies have been combined to design and construct a genome-wide, sequence-verified, arrayed CRISPR library. This resource allows single-target or combinatorial genetic screens to be carried out at scale in a multiplexed or arrayed format. By conducting parallel loss-of-function screens, we compare our approach to existing sgRNA design and expression strategies.
dc.description.sponsorshipThis work was supported in part by funding from CRUK.
dc.language.isoenen
dc.publisherElsevier (Cell Press)
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectCRISPRen
dc.subjectsgRNAen
dc.subjectalgorithmen
dc.subjectcombinatorialen
dc.subjectscreenen
dc.subjectexpressionen
dc.subjectCas9en
dc.subjectpredictionen
dc.subjectlentivirusen
dc.subjectlibraryen
dc.titleA CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockouten
dc.typeArticle
prism.endingPage354.e3
prism.issueIdentifier2en
prism.publicationDate2017en
prism.publicationNameMolecular Cellen
prism.startingPage348
prism.volume67en
dc.identifier.doi10.17863/CAM.11223
dcterms.dateAccepted2017-06-23en
rioxxterms.versionofrecord10.1016/j.molcel.2017.06.030en
rioxxterms.versionAMen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-07-20en
dc.contributor.orcidHannon, Gregory [0000-0003-4021-3898]
dc.identifier.eissn1097-4164
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idCancer Research UK (C14303/A17197)
pubs.funder-project-idPew Charitable Trusts (28354)
pubs.funder-project-idCancer Research UK (C14303_do not transfer)
cam.orpheus.successThu Jan 30 12:53:43 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International