Gain-of-function screen for genes that affect Drosophila muscle pattern formation.
Cohen, Stephen M
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Staudt, N., Molitor, A., Somogyi, K., Mata, J., Curado, S., Eulenberg, K., Meise, M., et al. (2005). Gain-of-function screen for genes that affect Drosophila muscle pattern formation.. PLoS genetics, 1 (4), e55. https://doi.org/10.1371/journal.pgen.0010055
This article reports the production of an EP-element insertion library with more than 3,700 unique target sites within the Drosophila melanogaster genome and its use to systematically identify genes that affect embryonic muscle pattern formation. We designed a UAS/GAL4 system to drive GAL4-responsive expression of the EP-targeted genes in developing apodeme cells to which migrating myotubes finally attach and in an intrasegmental pattern of cells that serve myotubes as a migration substrate on their way towards the apodemes. The results suggest that misexpression of more than 1.5% of the Drosophila genes can interfere with proper myotube guidance and/or muscle attachment. In addition to factors already known to participate in these processes, we identified a number of enzymes that participate in the synthesis or modification of protein carbohydrate side chains and in Ubiquitin modifications and/or the Ubiquitin-dependent degradation of proteins, suggesting that these processes are relevant for muscle pattern formation.
Muscles, Cytoskeleton, Animals, Drosophila melanogaster, Drosophila Proteins, Ubiquitin, Genetic Techniques, Cell Cycle, Cell Movement, Gene Expression Regulation, Developmental, Body Patterning, Genes, Insect, Muscle Fibers, Skeletal
External DOI: https://doi.org/10.1371/journal.pgen.0010055
This record's URL: https://www.repository.cam.ac.uk/handle/1810/265984
Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International