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dc.contributor.authorStaudt, Nicoleen
dc.contributor.authorMolitor, Andreasen
dc.contributor.authorSomogyi, Kalmanen
dc.contributor.authorMata, Juanen
dc.contributor.authorCurado, Silviaen
dc.contributor.authorEulenberg, Karstenen
dc.contributor.authorMeise, Martinen
dc.contributor.authorSiegmund, Thomasen
dc.contributor.authorHäder, Thomasen
dc.contributor.authorHilfiker, Andresen
dc.contributor.authorBrönner, Günteren
dc.contributor.authorEphrussi, Anneen
dc.contributor.authorRørth, Pernilleen
dc.contributor.authorCohen, Stephen Men
dc.contributor.authorFellert, Sonjaen
dc.contributor.authorChung, Ho-Ryunen
dc.contributor.authorPiepenburg, Olafen
dc.contributor.authorSchäfer, Ulrichen
dc.contributor.authorJäckle, Herberten
dc.contributor.authorVorbrüggen, Gerden
dc.date.accessioned2017-08-08T10:30:42Z
dc.date.available2017-08-08T10:30:42Z
dc.date.issued2005-10-28en
dc.identifier.issn1553-7404
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/265984
dc.description.abstractThis article reports the production of an EP-element insertion library with more than 3,700 unique target sites within the Drosophila melanogaster genome and its use to systematically identify genes that affect embryonic muscle pattern formation. We designed a UAS/GAL4 system to drive GAL4-responsive expression of the EP-targeted genes in developing apodeme cells to which migrating myotubes finally attach and in an intrasegmental pattern of cells that serve myotubes as a migration substrate on their way towards the apodemes. The results suggest that misexpression of more than 1.5% of the Drosophila genes can interfere with proper myotube guidance and/or muscle attachment. In addition to factors already known to participate in these processes, we identified a number of enzymes that participate in the synthesis or modification of protein carbohydrate side chains and in Ubiquitin modifications and/or the Ubiquitin-dependent degradation of proteins, suggesting that these processes are relevant for muscle pattern formation.
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectMusclesen
dc.subjectCytoskeletonen
dc.subjectAnimalsen
dc.subjectDrosophila melanogasteren
dc.subjectDrosophila Proteinsen
dc.subjectUbiquitinen
dc.subjectGenetic Techniquesen
dc.subjectCell Cycleen
dc.subjectCell Movementen
dc.subjectGene Expression Regulation, Developmentalen
dc.subjectBody Patterningen
dc.subjectGenes, Insecten
dc.subjectMuscle Fibers, Skeletalen
dc.titleGain-of-function screen for genes that affect Drosophila muscle pattern formation.en
dc.typeArticle
prism.issueIdentifier4en
prism.publicationDate2005en
prism.publicationNamePLoS geneticsen
prism.startingPagee55
prism.volume1en
dc.identifier.doi10.17863/CAM.12315
dcterms.dateAccepted2005-09-29en
rioxxterms.versionofrecord10.1371/journal.pgen.0010055en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2005-10-28en
dc.contributor.orcidMata, Juan [0000-0002-5514-3653]
dc.contributor.orcidEphrussi, Anne [0000-0002-5061-4620]
dc.identifier.eissn1553-7404
rioxxterms.typeJournal Article/Reviewen
rioxxterms.freetoread.startdate2016-10-28


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International