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dc.contributor.authorBetzer, Oen
dc.contributor.authorMeir, Ren
dc.contributor.authorDreifuss, Ten
dc.contributor.authorShamalov, Ken
dc.contributor.authorMotiei, Men
dc.contributor.authorShwartz, Aen
dc.contributor.authorBaranes, Kobyen
dc.contributor.authorCohen, CJen
dc.contributor.authorShraga-Heled, Nen
dc.contributor.authorOfir, Ren
dc.contributor.authorYadid, Gen
dc.contributor.authorPopovtzer, Ren
dc.date.accessioned2017-08-08T13:50:18Z
dc.date.available2017-08-08T13:50:18Z
dc.date.issued2015-10-28en
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/266044
dc.description.abstractRecent advances in theranostic nanomedicine can promote stem cell and immune cell-based therapy. Gold nanoparticles (GNPs) have been shown to be promising agents for in-vivo cell-tracking in cell-based therapy applications. Yet a crucial challenge is to develop a reliable protocol for cell upload with, on the one hand, sufficient nanoparticles to achieve maximum visibility of cells, while on the other hand, assuring minimal effect of particles on cell function and viability. Previous studies have demonstrated that the physicochemical parameters of GNPs have a critical impact on their efficient uptake by cells. In the current study we have examined possible variations in GNP uptake, resulting from different incubation period and concentrations in different cell-lines. We have found that GNPs effectively labeled three different cell-lines - stem, immune and cancer cells, with minimal impairment to cell viability and functionality. We further found that uptake efficiency of GNPs into cells stabilized after a short period of time, while GNP concentration had a significant impact on cellular uptake, revealing cell-dependent differences. Our results suggest that while heeding the slight variations within cell lines, modifying the loading time and concentration of GNPs, can promote cell visibility in various nanoparticle-dependent in-vivo cell tracking and imaging applications.
dc.description.sponsorshipIsrael Cancer Research Fund (ICRF), Israel Science Foundation (grant #749/14), Christians for Israel Chair in Medical Research
dc.languageengen
dc.language.isoenen
dc.publisherNature Publishing Group
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectCell Lineen
dc.subjectCell Survivalen
dc.subjectCell Trackingen
dc.subjectGolden
dc.subjectHumansen
dc.subjectMetal Nanoparticlesen
dc.subjectNeoplasmsen
dc.subjectStem Cellsen
dc.subjectT-Lymphocytesen
dc.subjectTheranostic Nanomedicineen
dc.titleIn-vitro Optimization of Nanoparticle-Cell Labeling Protocols for In-vivo Cell Tracking Applications.en
dc.typeArticle
prism.number15400en
prism.publicationDate2015en
prism.publicationNameScientific Reportsen
prism.volume5en
dc.identifier.doi10.17863/CAM.9617
dcterms.dateAccepted2015-09-22en
rioxxterms.versionofrecord10.1038/srep15400en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2015-10-28en
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Reviewen
cam.issuedOnline2015-10-28en


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International