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dc.contributor.authorBrowning, Hen
dc.contributor.authorHayles, Jen
dc.contributor.authorMata, Juanen
dc.contributor.authorAveline, Len
dc.contributor.authorNurse, Pen
dc.contributor.authorMcIntosh, JRen
dc.date.accessioned2017-08-09T12:56:10Z
dc.date.available2017-08-09T12:56:10Z
dc.date.issued2000-10-02en
dc.identifier.issn0021-9525
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/266117
dc.description.abstractCytoplasmic microtubules are critical for establishing and maintaining cell shape and polarity. Our investigations of kinesin-like proteins (klps) and morphological mutants in the fission yeast Schizosaccharomyces pombe have identified a kinesin-like gene, tea2(+), that is required for cells to generate proper polarized growth. Cells deleted for this gene are often bent during exponential growth and initiate growth from improper sites as they exit stationary phase. They have a reduced cytoplasmic microtubule network and display severe morphological defects in genetic backgrounds that produce long cells. The tip-specific marker, Tea1p, is mislocalized in both tea2-1 and tea2Delta cells, indicating that Tea2p function is necessary for proper localization of Tea1p. Tea2p is localized to the tips of the cell and in a punctate pattern within the cell, often coincident with the ends of cytoplasmic microtubules. These results suggest that this kinesin promotes microtubule growth, possibly through interactions with the microtubule end, and that it is important for establishing and maintaining polarized growth along the long axis of the cell.
dc.language.isoenen
dc.publisherRockefeller University Press
dc.subjectAmino Acid Sequence Cell Compartmentation Cell Polarity/*genetics Cloning, Molecular Fungal Proteins/genetics *Genes, Fungal Microtubule-Associated Proteins/*genetics Microtubules/*ultrastructure Moleen
dc.titleTea2p is a kinesin-like protein required to generate polarized growth in fission yeasten
dc.typeArticle
prism.endingPage15-28
prism.issueIdentifier1en
prism.publicationDate2000en
prism.publicationNameJournal of Cell Biologyen
prism.startingPage15-28
prism.volume151en
dc.identifier.doi10.17863/CAM.12314
dcterms.dateAccepted2000-08-17en
rioxxterms.versionofrecord10.1083/jcb.151.1.15en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2000-10-02en
dc.contributor.orcidMata, Juan [0000-0002-5514-3653]
dc.identifier.eissn1540-8140
rioxxterms.typeJournal Article/Reviewen


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