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dc.contributor.authorHe, Jayen
dc.contributor.authorCooper, HMen
dc.contributor.authorReyes Tellez, Aurelioen
dc.contributor.authorDi Re, Men
dc.contributor.authorKazak, Len
dc.contributor.authorWood, SRen
dc.contributor.authorMao, CCen
dc.contributor.authorFearnley, Ianen
dc.contributor.authorWalker, Johnen
dc.contributor.authorHolt, IJen
dc.date.accessioned2017-08-11T11:22:51Z
dc.date.available2017-08-11T11:22:51Z
dc.date.issued2012-07en
dc.identifier.issn0305-1048
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/266270
dc.description.abstractThe bacterial homologue of C4orf14, YqeH, has been linked to assembly of the small ribosomal subunit. Here, recombinant C4orf14 isolated from human cells, co-purified with the small, 28S subunit of the mitochondrial ribosome and the endogenous protein co-fractionated with the 28S subunit in sucrose gradients. Gene silencing of C4orf14 specifically affected components of the small subunit, leading to decreased protein synthesis in the organelle. The GTPase of C4orf14 was critical to its interaction with the 28S subunit, as was GTP. Therefore, we propose that C4orf14, with bound GTP, binds to components of the 28S subunit facilitating its assembly, and GTP hydrolysis acts as the release mechanism. C4orf14 was also found to be associated with human mitochondrial nucleoids, and C4orf14 gene silencing caused mitochondrial DNA depletion. In vitro C4orf14 is capable of binding to DNA. The association of C4orf14 with mitochondrial translation factors and the mitochondrial nucleoid suggests that the 28S subunit is assembled at the mitochondrial nucleoid, enabling the direct transfer of messenger RNA from the nucleoid to the ribosome in the organelle.
dc.description.sponsorshipMedical Research Council (MRC); Biotechnology and Biological Sciences Research Council (BBSRC); European Union; Academy of Finland (to H.M.C.). Funding for open access charge: MRC.
dc.languageengen
dc.language.isoenen
dc.publisherOxford University Press
dc.rightsAttribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectCell Line, Tumoren
dc.subjectDNA, Mitochondrialen
dc.subjectGTP Phosphohydrolasesen
dc.subjectGuanosine Triphosphateen
dc.subjectHEK293 Cellsen
dc.subjectHumansen
dc.subjectMitochondriaen
dc.subjectRibosome Subunits, Small, Eukaryoticen
dc.titleHuman C4orf14 interacts with the mitochondrial nucleoid and is involved in the biogenesis of the small mitochondrial ribosomal subunit.en
dc.typeArticle
prism.endingPage6108
prism.issueIdentifier13en
prism.publicationDate2012en
prism.publicationNameNucleic Acids Researchen
prism.startingPage6097
prism.volume40en
dc.identifier.doi10.17863/CAM.9970
dcterms.dateAccepted2012-03-05en
rioxxterms.versionofrecord10.1093/nar/gks257en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by-nc/4.0/en
rioxxterms.licenseref.startdate2012-07en
dc.contributor.orcidReyes Tellez, Aurelio [0000-0003-2876-2202]
dc.contributor.orcidWalker, John [0000-0001-7929-2162]
dc.identifier.eissn1362-4962
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMRC (MC_U105663148)
cam.issuedOnline2012-07-01en
dc.identifier.urlhttps://academic.oup.com/nar/article/40/13/6097/1012380#83691234en


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Attribution-NonCommercial 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial 4.0 International