Mitochondrial nucleoid interacting proteins support mitochondrial protein synthesis.
Di Re, M
Nucleic Acids Research
Oxford University Press
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He, J., Cooper, H., Reyes Tellez, A., Di Re, M., Sembongi, H., Litwin, T., Gao, J., et al. (2012). Mitochondrial nucleoid interacting proteins support mitochondrial protein synthesis.. Nucleic Acids Research, 40 (13), 6109-6121. https://doi.org/10.1093/nar/gks266
Mitochondrial ribosomes and translation factors co-purify with mitochondrial nucleoids of human cells, based on affinity protein purification of tagged mitochondrial DNA binding proteins. Among the most frequently identified proteins were ATAD3 and prohibitin, which have been identified previously as nucleoid components, using a variety of methods. Both proteins are demonstrated to be required for mitochondrial protein synthesis in human cultured cells, and the major binding partner of ATAD3 is the mitochondrial ribosome. Altered ATAD3 expression also perturbs mtDNA maintenance and replication. These findings suggest an intimate association between nucleoids and the machinery of protein synthesis in mitochondria. ATAD3 and prohibitin are tightly associated with the mitochondrial membranes and so we propose that they support nucleic acid complexes at the inner membrane of the mitochondrion.
ATPases Associated with Diverse Cellular Activities, Adenosine Triphosphatases, Cell Cycle Proteins, Cell Line, Tumor, DNA, Mitochondrial, HEK293 Cells, Humans, Membrane Proteins, Mitochondria, Mitochondrial Proteins, Nuclear Proteins, Nucleoproteins, Protein Biosynthesis, RNA, RNA, Messenger, RNA, Mitochondrial, Repressor Proteins, Ribosomes
Medical Research Council (MRC) and the Biotechnology and Biological Sciences Research Council (BBSRC); Intramural program of the National Institutes of Health, National Heart, Lung and Blood Institute (to K.N.); Academy of Finland (to H.M.C.). Funding for open access charge: MRC.
External DOI: https://doi.org/10.1093/nar/gks266
This record's URL: https://www.repository.cam.ac.uk/handle/1810/266271
Attribution-NonCommercial 4.0 International
Licence URL: http://creativecommons.org/licenses/by-nc/4.0/