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dc.contributor.authorHe, Jayen
dc.contributor.authorCooper, HMen
dc.contributor.authorReyes Tellez, Aurelioen
dc.contributor.authorDi Re, Men
dc.contributor.authorSembongi, Hiroshien
dc.contributor.authorLitwin, TRen
dc.contributor.authorGao, Jen
dc.contributor.authorNeuman, KCen
dc.contributor.authorFearnley, Ianen
dc.contributor.authorSpinazzola, Aen
dc.contributor.authorWalker, Johnen
dc.contributor.authorHolt, IJen
dc.date.accessioned2017-08-11T11:35:18Z
dc.date.available2017-08-11T11:35:18Z
dc.date.issued2012-07en
dc.identifier.issn0305-1048
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/266271
dc.description.abstractMitochondrial ribosomes and translation factors co-purify with mitochondrial nucleoids of human cells, based on affinity protein purification of tagged mitochondrial DNA binding proteins. Among the most frequently identified proteins were ATAD3 and prohibitin, which have been identified previously as nucleoid components, using a variety of methods. Both proteins are demonstrated to be required for mitochondrial protein synthesis in human cultured cells, and the major binding partner of ATAD3 is the mitochondrial ribosome. Altered ATAD3 expression also perturbs mtDNA maintenance and replication. These findings suggest an intimate association between nucleoids and the machinery of protein synthesis in mitochondria. ATAD3 and prohibitin are tightly associated with the mitochondrial membranes and so we propose that they support nucleic acid complexes at the inner membrane of the mitochondrion.
dc.description.sponsorshipMedical Research Council (MRC) and the Biotechnology and Biological Sciences Research Council (BBSRC); Intramural program of the National Institutes of Health, National Heart, Lung and Blood Institute (to K.N.); Academy of Finland (to H.M.C.). Funding for open access charge: MRC.
dc.languageengen
dc.language.isoenen
dc.publisherOxford University Press
dc.rightsAttribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectATPases Associated with Diverse Cellular Activitiesen
dc.subjectAdenosine Triphosphatasesen
dc.subjectCell Cycle Proteinsen
dc.subjectCell Line, Tumoren
dc.subjectDNA, Mitochondrialen
dc.subjectHEK293 Cellsen
dc.subjectHumansen
dc.subjectMembrane Proteinsen
dc.subjectMitochondriaen
dc.subjectMitochondrial Proteinsen
dc.subjectNuclear Proteinsen
dc.subjectNucleoproteinsen
dc.subjectProtein Biosynthesisen
dc.subjectRNAen
dc.subjectRNA, Messengeren
dc.subjectRNA, Mitochondrialen
dc.subjectRepressor Proteinsen
dc.subjectRibosomesen
dc.titleMitochondrial nucleoid interacting proteins support mitochondrial protein synthesis.en
dc.typeArticle
prism.endingPage6121
prism.issueIdentifier13en
prism.publicationDate2012en
prism.publicationNameNucleic Acids Researchen
prism.startingPage6109
prism.volume40en
dc.identifier.doi10.17863/CAM.9969
dcterms.dateAccepted2012-03-10en
rioxxterms.versionofrecord10.1093/nar/gks266en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2012-07en
dc.contributor.orcidReyes Tellez, Aurelio [0000-0003-2876-2202]
dc.contributor.orcidWalker, John [0000-0001-7929-2162]
dc.identifier.eissn1362-4962
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMRC (MC_U105663148)
cam.issuedOnline2012-07-01en
dc.identifier.urlhttps://academic.oup.com/nar/article/40/13/6109/1014562#83695995en


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Attribution-NonCommercial 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial 4.0 International