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dc.contributor.authorYing, Q-Len
dc.contributor.authorSmith, Austinen
dc.date.accessioned2017-08-15T11:38:44Z
dc.date.available2017-08-15T11:38:44Z
dc.date.issued2017-06-06en
dc.identifier.issn2213-6711
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/266393
dc.description.abstractEmbryonic stem cells (ESCs) are a unique tool for genetic perturbation of mammalian cellular and organismal processes additionally in humans offer unprecedented opportunities for disease modeling and cell therapy. Furthermore, ESCs are a powerful system for exploring the fundamental biology of pluripotency. Indeed understanding the control of self-renewal and differentiation is key to realizing the potential of ESCs. Building on previous observations, we found that mouse ESCs can be derived and maintained with high efficiency through insulation from differentiation cues combined with consolidation of an innate cell proliferation program. This finding of a pluripotent ground state has led to conceptual and practical advances, including the establishment of germline-competent ESCs from recalcitrant mouse strains and for the first time from the rat. Here, we summarize historical and recent progress in defining the signaling environment that supports self-renewal. We compare the contrasting requirements of two types of pluripotent stem cell, naive ESCs and primed post-implantation epiblast stem cells (EpiSCs), and consider the outstanding challenge of generating naive pluripotent stem cells from different mammals.
dc.description.sponsorshipResearch in the Q.-L.Y. laboratory is supported by the NIH (R01 OD010926), the California Institute for Regener ative Medicine (RN2-00938, RS1-00327, and RT3-07949), and the Yong Chen Foundation of the Zhongmei Group. A.S. is a Medical Research Council Professor and receives research funding from the Medical Research Council (MR/P00072X/1) and the Biotechnology and Biological Sciences Research Council of the United Kingdom (BB/P009867/1), and from the European Commission (PluriMes Project no. 602423).
dc.languageengen
dc.language.isoenen
dc.publisherElsevier
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectERKen
dc.subjectLIF/Stat3en
dc.subjectMEKen
dc.subjectWnt/β-cateninen
dc.subjectembryonic stem cellen
dc.subjectepiblast stem cellen
dc.subjectnaive pluripotencyen
dc.subjectprimed pluripotencyen
dc.subjectstem cell differentiationen
dc.subjectstem cell self-renewalen
dc.titleThe Art of Capturing Pluripotency: Creating the Right Cultureen
dc.typeArticle
prism.endingPage1464
prism.issueIdentifier6en
prism.publicationDate2017en
prism.publicationNameStem Cell Reportsen
prism.startingPage1457
prism.volume8en
dc.identifier.doi10.17863/CAM.12617
dcterms.dateAccepted2017-05-17en
rioxxterms.versionofrecord10.1016/j.stemcr.2017.05.020en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-06-06en
dc.contributor.orcidSmith, Austin [0000-0002-3029-4682]
dc.identifier.eissn2213-6711
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/P009867/1)
pubs.funder-project-idMRC (MR/P00072X/1)
pubs.funder-project-idEuropean Commission (602423)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International