"Pathotyping" Multiplex PCR Assay for Haemophilus parasuis: a Tool for Prediction of Virulence.
Peters, Sarah E
Williamson, Susanna M
Rycroft, Andrew N
Wren, Brendan W
Journal of clinical microbiology
American Society for Microbiology
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Howell, K., Weinert, L., Peters, S. E., Wang, J., Hernandez-Garcia, J., Chaudhuri, R. R., Luan, S., et al. (2017). "Pathotyping" Multiplex PCR Assay for Haemophilus parasuis: a Tool for Prediction of Virulence.. Journal of clinical microbiology, 55 (9), 2617-2628. https://doi.org/10.1128/jcm.02464-16
Haemophilus parasuis is a diverse bacterial species found in the upper respiratory tract of pigs that can also cause Glässer's disease and pneumonia. A previous pan-genome study of H. parasuis identified 48 genes that were associated with clinical disease. Here we describe the development of a generalised linear model (termed a pathotyping model) to predict the potential virulence of isolates of H. parasuis, based on a subset of 10 genes from the pan-genome. A multiplex PCR (mPCR) was constructed based on these genes, the results of which were entered into the pathotyping model to yield a prediction of virulence. This new diagnostic mPCR was tested on 143 field isolates of H. parasuis that had previously been whole-genome sequenced and a further 84 isolates from the UK from cases of H. parasuis-related disease in pigs collected between 2013 and 2014. The combination of the mPCR and the pathotyping model predicted the virulence of an isolate with 78% accuracy for the original isolate collection, and 90% for the additional isolate collection, providing an overall accuracy of 83% (81% sensitivity and 93% specificity) when compared with the 'current standard' of detailed clinical meta-data. This new pathotyping assay has the potential to aid surveillance and disease control in addition to serotyping data.
Animals, Swine, Haemophilus parasuis, Haemophilus Infections, Swine Diseases, Molecular Diagnostic Techniques, Virulence, Genome, Multiplex Polymerase Chain Reaction
Royal Society (DH140195)
WELLCOME TRUST (109385/Z/15/Z)
External DOI: https://doi.org/10.1128/jcm.02464-16
This record's URL: https://www.repository.cam.ac.uk/handle/1810/266712