Research Data: Membrane Adhesion through Bridging by Multimeric Ligands

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Authors
Amjad, Omar
Mognetti, BM
  Cicuta, Pietro
  Di Michele, Lorenzo
Publication Date
2017-08-22
Type
Dataset
Metadata
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Citation
Amjad, O., Mognetti, B., Cicuta, P., & Di Michele, L. (2017). Research Data: Membrane Adhesion through Bridging by Multimeric Ligands [Dataset]. https://doi.org/10.17863/CAM.6919
Description
File formatt is .tiff, with each .tiff file representing a single frame of video or slice of stack. Individual vesicles are organised into .zip files corresponding to their SA/DNA concentration ratios and experiment type: Z-Stack, Flickering or FRAP. In the Z-stacks .zip files, two channels were recorded, using a 488nm and 633nm laser respectively. For each vesicle folder, there is a separate subfolder for each of these channels channel. Each frame of the Z-stacks represents a 0.1um slice. In Flickering videos, each .tiff file is a frame of a 1000 frame video, with a framerate of 97ms/frame, taken at the equator of the vesicle. In FRAP experiments, each vesicle subfolder contains subfolders at different frame rates. These are: StagePre (10fps), StagePost1 (10fps), StagePost2 (0.5fps) and StagePost3 (0.1fps). These correspond to stages of the pre- and post-bleaching process.
Format
Matlab, ImageJ
Keywords
Lipid Membranes, Membrane Adhesion, Multivalent Interactions, Biotin-Streptavidin, Biosensing
Relationships
Publication Reference: https://doi.org/10.1021/acs.langmuir.6b03692
Sponsorship
EPSRC (1503634)
EPSRC (via Imperial College London) (CHIS_P39012)
Leverhulme Trust (ECF-2015-494)
Isaac Newton Trust (MIN 1508(S))
Identifiers
This record's DOI: https://doi.org/10.17863/CAM.6919

Rights
Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International
Licence URL: http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International