Serotonergic modulation of the activity of GLP-1 producing neurons in the nucleus of the solitary tract in mouse.
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Holt, M., Llewellyn-Smith, I., Reimann, F., Gribble, F., & Trapp, S. (2017). Serotonergic modulation of the activity of GLP-1 producing neurons in the nucleus of the solitary tract in mouse.. Molecular Metabolism, 6 (8), 909-921. https://doi.org/10.1016/j.molmet.2017.06.002
OBJECTIVE: Glucagon-like peptide-1 (GLP-1) and 5-HT are potent regulators of food intake within the brain. GLP-1 is expressed by preproglucagon (PPG) neurons in the nucleus tractus solitarius (NTS). We have previously shown that PPG neurons innervate 5-HT neurons in the ventral brainstem. Here, we investigate whether PPG neurons receive serotonergic input and respond to 5-HT. METHODS: We employed immunohistochemistry to reveal serotonergic innervation of PPG neurons. We investigated the responsiveness of PPG neurons to 5-HT using in vitro Ca²⁺ imaging in brainstem slices from transgenic mice expressing the Ca²⁺ indicator, GCaMP3, in PPG neurons, and cell-attached patch-clamp recordings. RESULTS: Close appositions from 5-HT-immunoreactive axons occurred on many PPG neurons. Application of 20 μM 5-HT produced robust Ca²⁺ responses in NTS PPG dendrites but little change in somata. Dendritic Ca²⁺ spikes were concentration-dependent (2, 20, and 200 μM) and unaffected by blockade of glutamatergic transmission, suggesting 5-HT receptors on PPG neurons. Neither activation nor blockade of 5-HT₃ receptors affected [Ca²⁺]i. In contrast, inhibition of 5-HT₃ receptors attenuated increases in intracellular Ca²⁺ and 5-HT₂c receptor activation produced Ca²⁺ spikes. Patch-clamp recordings revealed that 44% of cells decreased their firing rate under 5-HT, an effect blocked by 5-HT₁ᴀ receptor antagonism. CONCLUSIONS: PPG neurons respond directly to 5-HT with a 5-HT₂c receptor-dependent increase in dendritic [Ca²⁺]i. Electrical responses to 5-HT revealed additional inhibitory effects due to somatic 5-HT₁ᴀ receptors. Reciprocal innervation between 5-HT and PPG neurons suggests that the coordinated activity of these brainstem neurons may play a role in the regulation of food intake.
Serotonin, Preproglucagon, GCaMP, Dendritic calcium, NTS, nucleus tractus solitarius
This study was supported by grants MR/J013293/2 from the MRC, UK (ST) and Project Grant #1025031 from NHMRC Australia (ILS). MKH holds a UCL Graduate Research Scholarship. FR and FMG are supported by the Wellcome Trust (106262/Z/14/Z, 106263/Z/14/Z) and the MRC (MRC_MC_UU_12012/3).
WELLCOME TRUST (106262/Z/14/Z & 106263/Z/14/Z)
External DOI: https://doi.org/10.1016/j.molmet.2017.06.002
This record's URL: https://www.repository.cam.ac.uk/handle/1810/266994
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