Collagen-Fibrinogen Lyophilised Scaffolds for Soft Tissue Regeneration
Multidisciplinary Publishing Institute
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Shepherd, J., Bax, D., Best, S., & Cameron, R. (2017). Collagen-Fibrinogen Lyophilised Scaffolds for Soft Tissue Regeneration. Materials, 10 (6. 568)https://doi.org/10.3390/ma10060568
A significant body of research has considered collagen as a scaffold material for soft tissue regeneration. The main structural component of extra-cellular matrix (ECM), collagen’s advantages over synthetic polymers are numerous. However, for applications where higher stiffness and stability are required, significant cross-linking may affect bioactivity. A carbodiimide (EDC) cross-linking route consumes carboxylate groups that are key to collagen’s essential cell recognition motifs (GxOGER). Fibrinogen was considered as a promising additive as it plays a key role in the process of wound repair and contains RGD integrin binding sites which bind to a variety of cells, growth factors and cytokines. Fibrinogen’s binding sites however, also contain the same carboxylate groups as collagen. We have successfully produced highly interconnected, porous collagen-fibrinogen scaffolds using a lyophilisation technique and micro-computed tomography demonstrated minimal influence of either fibrinogen content or cross-linking concentration on the scaffold structure. The specific biological effect of fibrinogen additions into cross-linked collagen are considered by using films as a model for the struts of bulk scaffolds. By considering various additions of fibrinogen to the collagen film with increasing degrees of cross-linking, this study demonstrates a significant biological advantage with fibrinogen addition across the cross-linking concentrations typically applied to collagen-based scaffolds.
collagen, fibrinogen, adhesion, lyophilisation, micro-computed tomography
Is supplemented by: https://doi.org/10.17863/CAM.9603
The authors wish to acknowledge the support of the European Research Council for the Advanced Grant 320598 awarded to REC and the EPSRC for the Established Career Fellowship EP/N019938/1 awarded to REC and SMB. They also wish to acknowledge funding for DB from the Peoples Programme of the EU 7th Framework Programme (RAE no: PHF-GA-2013-624904).
European Research Council (320598)
External DOI: https://doi.org/10.3390/ma10060568
This record's URL: https://www.repository.cam.ac.uk/handle/1810/267037
Attribution 4.0 International
Licence URL: http://creativecommons.org/licenses/by/4.0/