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dc.contributor.authorItzhaki, LSR
dc.contributor.authorPerez-Riba, A
dc.date.accessioned2017-09-06T13:21:28Z
dc.date.available2017-09-06T13:21:28Z
dc.date.issued2017-08-22
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/267062
dc.description.abstractQuantitative determination of protein thermodynamic stability is fundamental to many research areas, both basic and applied. Although chemical-induced denaturation is the gold-standard method, it has been replaced in many settings by semi-quantitative approaches such as thermal stability measurements. The reason for this shift is that chemical denaturation experiments are labour-intensive, sample-costly and time-consuming, and it has been assumed that miniaturisation to a high-throughput format would not be possible without concomitantly comprising data quality. Here we exploit current technologies to create a high-throughput label-free chemical denaturation method that is capable of generating replicate datasets on multiple proteins in parallel on a timescale that is at least ten times faster, much more economical on sample, and with the potential for superior data quality, than the conventional methods used in most research labs currently.
dc.language.isoen
dc.publisherNature Publishing Group
dc.rightsAttribution 4.0 International
dc.rightsAttribution 4.0 International
dc.rightsAttribution 4.0 International
dc.rightsAttribution 4.0 International
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectassay systems
dc.subjectexpression systems
dc.subjectthermodynamics
dc.titleA method for rapid high-throughput biophysical analysis of proteins
dc.typeArticle
prism.number9071
prism.publicationNameScientific Reports
prism.volume7
dc.identifier.doi10.17863/CAM.12164
dcterms.dateAccepted2017-07-14
rioxxterms.versionofrecord10.1038/s41598-017-08664-w
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.licenseref.startdate2017-07-14
dc.contributor.orcidItzhaki, Laura [0000-0001-6504-2576]
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Review
pubs.funder-project-idCancer Research UK (22676)
cam.issuedOnline2017-08-22
cam.orpheus.successThu Jan 30 13:00:24 GMT 2020 - The item has an open VoR version.
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International