Human mitochondrial ribosomes can switch structural tRNAs - but when and why?
Taylor & Francis
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Chrzanowska-Lightowlers, Z., Rorbach, J., & Minczuk, M. (2017). Human mitochondrial ribosomes can switch structural tRNAs - but when and why?. RNA Biology, 0-0. https://doi.org/10.1080/15476286.2017.1356551
High resolution cryoEM of mammalian mitoribosomes revealed the unexpected presence of mitochondrially encoded tRNA as a structural component of mitochondrial large ribosomal subunit (mt-LSU). Our previously published data identified that only mitochondrial (mt-) tRNA(Phe) and mt-tRNA(Val) can be incorporated into mammalian mt-LSU and within an organism there is no evidence of tissue specific variation. When mt-tRNA(Val) is limiting, human mitoribosomes can integrate mt-tRNA(Phe) instead to generate a translationally competent monosome. Here we discuss the possible reasons for and consequences of the observed plasticity of the structural mt-tRNA integration. We also indicate potential direction for further research that could help our understanding of the mechanistic and evolutionary aspects of this unprecedented system.
mitochondria, human, mammalian, rRNA, ribosomes, tRNA
This work was supported by the Wellcome Trust under Grant 096919/Z/11/Z and Medical Research Council UK under Grant MC_U105697135.
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External DOI: https://doi.org/10.1080/15476286.2017.1356551
This record's URL: https://www.repository.cam.ac.uk/handle/1810/267382