The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules
Rebelo Guiomar, Pedro
Journal of Biological Chemistry
American Society for Biochemistry and Molecular Biology Inc.
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Zaganelli, S., Rebelo Guiomar, P., Maundrell, K., Rozanska, A., Pierredon, S., Powell, C., Jourdain, A., et al. (2017). The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules. Journal of Biological Chemistry, 292 (11), 4519-4532. https://doi.org/10.1074/jbc.M116.771105
Mitochondrial gene expression is a fundamental process that is largely dependent on nuclear-encoded proteins. Several steps of mitochondrial RNA processing and maturation, including RNA post-transcriptional modification, appear to be spatially organized into distinct foci, which we have previously termed mitochondrial RNA granules (MRGs). Although an increasing number of proteins have been localized to MRGs, a comprehensive analysis of the proteome of these structures is still lacking. Here, we have applied a microscopy-based approach that has allowed us to identify novel components of the MRG proteome. Among these, we have focused our attention on RPUSD4, an uncharacterized mitochondrial putative pseudouridine synthase. We show that RPUSD4 depletion leads to a severe reduction of the steady-state level of the 16S mitochondrial (mt) rRNA with defects in the biogenesis of the mitoribosome large subunit and consequently in mitochondrial translation. We report that RPUSD4 binds 16S mt-rRNA, mt-tRNA(Met), and mt-tRNA(Phe), and we demonstrate that it is responsible for pseudouridylation of the latter. These data provide new insights into the relevance of RNA pseudouridylation in mitochondrial gene expression.
mitochondria, ribosome, RNA, RNA-binding protein, RNA modification, mitoribosome biogenesis, OXPHOS, pseudouridine, RPUSD
This work was supported by the Swiss National Science Foundation Grant SNF310030B_160257/1, Wellcome Trust Grant 096919/Z/11/Z, and core funding of Medical Research Council, UK.
Medical Research Council (MC_U105697135)
Medical Research Council (MC_UU_00015/4)
External DOI: https://doi.org/10.1074/jbc.M116.771105
This record's URL: https://www.repository.cam.ac.uk/handle/1810/267433
Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International
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