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Complete genome sequences of two insect-specific flaviviruses

Published version
Peer-reviewed

Type

Article

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Authors

Charles, J 
Tangudu, CS 
Firth, AE 
Blitvich, BJ 

Abstract

We determined the complete genomic sequences of two previously discovered insect-specific flaviviruses, Marisma mosquito virus (MMV) and Nanay virus (NANV), using a combination of high-throughput sequencing, reverse transcription-polymerase chain reaction, 5′ and 3′ rapid amplification of cDNA ends and Sanger sequencing. Complete polyprotein amino acid sequence alignments revealed that the closest known relatives of MMV and NANV are Donggang virus (89% identity, 95% similarity) and Nounané virus (53% identity, 70% similarity), respectively. Phylogenetic inference is in agreement with these findings. Potential programmed −1 ribosomal frameshifting sites were bioinformatically identified in the genomes of both viruses.

Description

Keywords

Animals, Flavivirus, Frameshifting, Ribosomal, Genome, Viral, High-Throughput Nucleotide Sequencing, Insecta, Phylogeny, Polyproteins, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Viral Proteins

Journal Title

Archives of Virology

Conference Name

Journal ISSN

0304-8608
1432-8798

Volume Title

Publisher

Springer Nature
Sponsorship
Wellcome Trust (106207/Z/14/Z)
European Research Council (646891)
This study was supported by a grant from the National Institutes of Health (R01AI114720). A.E.F. is supported by a Wellcome Trust grant (106207) and a European Research Council (ERC) European Union’s Horizon 2020 research and innovation programme grant (646891).