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dc.contributor.authorCarrington, Marken
dc.date.accessioned2017-10-02T16:38:49Z
dc.date.available2017-10-02T16:38:49Z
dc.date.issued2017-08-12en
dc.identifier.issn1759-6653
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/267509
dc.description.abstractThere are hundreds of Trypanosoma species that live in the blood and tissue spaces of their vertebrate hosts. The vast majority of these do not have the ornate system of antigenic variation that has evolved in the small number of African trypanosome species, but can still maintain long-term infections in the face of the vertebrate adaptive immune system. Trypanosoma theileri is a typical example, has a restricted host range of cattle and other Bovinae, and is only occasionally reported to cause patent disease although no systematic survey of the effect of infection on agricultural productivity has been performed. Here, a detailed genome sequence and a transcriptome analysis of gene expression in bloodstream form T. theileri have been performed. Analysis of the genome sequence and expression showed that T. theileri has a typical kinetoplastid genome structure and allowed a prediction that it is capable of meiotic exchange, gene silencing via RNA interference and, potentially, density-dependent growth control. In particular, the transcriptome analysis has allowed a comparison of two distinct trypanosome cell surfaces, T. brucei and T. theileri, that have each evolved to enable the maintenance of a long-term extracellular infection in cattle. The T. theileri cell surface can be modeled to contain a mixture of proteins encoded by four novel large and divergent gene families and by members of a major surface protease gene family. This surface composition is distinct from the uniform variant surface glycoprotein coat on African trypanosomes providing an insight into a second mechanism used by trypanosome species that proliferate in an extracellular milieu in vertebrate hosts to avoid the adaptive immune response.
dc.description.sponsorshipThis work was supported by the Biotechnology and Biological Sciences Research Council (BB/F00057X/1 and BB/L02442X/1 to K.M.); the Wellcome Trust grants (103740 and 095831 to K.M., 085256 to M.Ca.); an EU Horizon 2020 award (637765 to S.K.). S.K. is a Royal Society University Research Fellow; M.Cl. and K.T. are recipients of an RCUK-CONFAP research partnership award (BB/M029239/1). Next-generation sequencing and library construction was delivered via the BBSRC National Capability in Genomics (BB/J010375/1) at the Earlham Institute (EI, formerly The Genome Analysis Centre, Norwich), by members of the Platforms and Pipelines Group. Bioinformatics support was funded via BBSRC Institute Strategic Programme grant (BB/J004669/1) to the Earlham Institute.
dc.publisherOxford University Press
dc.rightsAttribution 4.0 International*
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectTrypanosoma theilerien
dc.subjectgenomeen
dc.subjecttranscriptomeen
dc.subjectcell surface componentsen
dc.titleAn Alternative Strategy for Trypanosome Survival in the Mammalian Bloodstream Revealed through Genome and Transcriptome Analysis of the Ubiquitous Bovine Parasite Trypanosoma (Megatrypanum) theilerien
dc.typeArticle
prism.endingPage2109
prism.issueIdentifier8en
prism.publicationDate2017en
prism.publicationNameGenome Biology and Evolutionen
prism.startingPage2093
prism.volume9en
dc.identifier.doi10.17863/CAM.13448
dcterms.dateAccepted2017-08-12en
rioxxterms.versionofrecord10.1093/gbe/evx152en
rioxxterms.versionVoR*
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-08-12en
dc.contributor.orcidCarrington, Mark [0000-0002-6435-7266]
dc.identifier.eissn1759-6653
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (085256/Z/08/Z)
cam.issuedOnline2017-08-14en
datacite.issupplementedby.doi10.5281/zenodo.193020en


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International