The most direct probe of non-Gaussian initial conditions has come from bispectrum measurements of temperature fluctuations in the Cosmic Microwave Background and of the matter and galaxy distribution at large scales. Such bispectrum estimators are expected to continue to provide the best constraints on the non-Gaussian parameters in future observations. We review and compare the theoretical and observational problems, current results and future prospects for the detection of a non-vanishing primordial component in the bispectrum of the Cosmic Microwave Background and large-scale structure, and the relation to specific predictions from different inflationary models.