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dc.contributor.authorDrechsler, Men
dc.contributor.authorGiavazzi, Fen
dc.contributor.authorCerbino, Ren
dc.contributor.authorPalacios De Castro, Isabelen
dc.date.accessioned2017-10-17T16:17:57Z
dc.date.available2017-10-17T16:17:57Z
dc.identifier.issn2041-1723
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/267869
dc.description.abstractTransport in cells occurs via a delicate interplay of passive and active processes, including diffusion, directed transport and advection. Despite progress in super-resolution microscopy, discriminating and quantifying these processes is a challenge, requiring tracking of rapidly moving, sub-diffraction objects in a crowded, noisy environment. Here we use Differential Dynamic Microscopy with different contrast mechanisms to provide a thorough characterization of the dynamics in the Drosophila oocyte. We study the movement of vesicles and the elusive motion of a cytoplasmic F-actin mesh, a known regulator of cytoplasmic flows. We find that cytoplasmic motility constitutes a combination of directed motion and random diffusion. While advection is mainly attributed to microtubules, we find that active diffusion is driven by the actin cytoskeleton, although it is also enhanced by the flow. We also find that an important dynamic link exists between vesicles and cytoplasmic F-actin motion, as recently suggested in mouse oocytes.
dc.description.sponsorshipMD and IMP were supported by the BBSRC, the Department of Zoology (Cambridge), the University of Cambridge, and an Isaac Newton Trust fellowship to MD. FG and RC acknowledge funding by the Italian Ministry of Education and Research, Futuro in Ricerca Project ANISOFT (RBFR125H0M) and by Fondazione CARIPLO-Regione Lombardia Project Light for Life (2016-0998).
dc.publisherNature Publishing Group
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectself-organizationen
dc.subjectactive processesen
dc.subjectmotionen
dc.subjectbody planen
dc.subjectoogenesisen
dc.subjectcell asymmetriesen
dc.subjectcytoskeletonen
dc.subjectmicrotubulesen
dc.subjectactinen
dc.subjectmotor proteinsen
dc.titleActive diffusion and advection in Drosophila oocytes result from the interplay of actin and microtubulesen
dc.typeArticle
prism.number1520en
prism.publicationNameNature Communicationsen
prism.volume8en
dc.identifier.doi10.17863/CAM.13790
dcterms.dateAccepted2017-09-14en
rioxxterms.versionofrecord10.1038/s41467-017-01414-6en
rioxxterms.versionVoR*
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-09-14en
dc.identifier.eissn2041-1723
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/L001748/1)
cam.issuedOnline2017-11-15en
cam.orpheus.counter1*


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International