Research Data: The role of optical projection in the analysis of membrane fluctuations
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Rautu, S., Orsi, D., Di Michele, L., Rowlands, G., Cicuta, P., & Turner, M. (2017). Research Data: The role of optical projection in the analysis of membrane fluctuations [Dataset]. https://doi.org/10.17863/CAM.9209
In the zipped folder "codes.zip" and sub-folders: flickering.m : matlab class that contains all the functions to set up and perform the analysis of a video of a flickering vesicle, to retrieve the spectrum of its fluctuations. example.m : a brief example script where the flickering.m class is used. export_spectrum.m : a scripts that loads the output of a flickering analysis (e.g. of example.m) and converts it to a json file format, ready for the fitting routine. DataAnalysis.nb : commented Mathematica notebook containing the fitting routine and an example of its usage. LTable.nb : support Mathematica file DataExample_X.json : example spectra readable by the Mathematica notebook. In the zipped folder "Sample_Data.zip": Examples of flickering confocal videos collected for the same vesicle at different pinhole size (and thus z-resolution, see paper). The videos are in tif format. The text file "Experimental" contains information on the pixel size, framerate, and pinhole size of each video.
See comments on the files. Software: Mathematica and Matlab for the analysis code. Most commercial image visualisation softwares can open tif files.
Flickering spectroscopy, Lipid membranes, Bending rigidity
Publication Reference: https://doi.org/10.1039/C7SM00108H
EPSRC (via Imperial College London) (CHIS_P39012)
Leverhulme Trust (ECF-2015-494)
Isaac Newton Trust (MIN 1508(S))
This record's DOI: https://doi.org/10.17863/CAM.9209
Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International, Attribution 4.0 International