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dc.contributor.authorAl-Lamki, Rafiaen
dc.contributor.authorBradley, Johnen
dc.contributor.authorPober, JSen
dc.date.accessioned2017-11-13T18:19:05Z
dc.date.available2017-11-13T18:19:05Z
dc.identifier.issn2296-858X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/269031
dc.description.abstractHuman studies, critical for developing new diagnostics and therapeutics, are limited by ethical and logistical issues, and preclinical animal studies are often poor predictors of human responses. Standard human cell cultures can address some of these concerns but the absence of the normal tissue microenvironment can alter cellular responses. Three-dimensional cultures that position cells on synthetic matrices, or organoid or organ-on-a-chip cultures, in which different cell spontaneously organize contacts with other cells and natural matrix only partly overcome this limitation. Here, we review how human organ cultures (HOCs) can more faithfully preserve in vivo tissue architecture and can better represent disease-associated changes. We will specifically describe how HOCs can be combined with both traditional and more modern morphological techniques to reveal how anatomic location can alter cellular responses at a molecular level and permit comparisons among different cells and different cell types within the same tissue. Examples are provided involving use of HOCs to study inflammation, cancer, and stem cell biology.
dc.description.sponsorshipThe authors would like to express their gratitude to The National Institute for Health Research (NIHR) Cambridge Biomedical Research Centre (RA-L, JB).
dc.publisherFrontiers Media
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjecthumanen
dc.subjectorgan cultureen
dc.subjectinflammationen
dc.subjectcanceren
dc.subjectstem cellsen
dc.titleHuman Organ Culture: Updating the Approach to Bridge the Gap from In Vitro to In Vivo in Inflammation, Cancer, and Stem Cell Biologyen
dc.typeArticle
prism.number148en
prism.publicationNameFrontiers in Medicineen
prism.volume4en
dc.identifier.doi10.17863/CAM.15238
dcterms.dateAccepted2017-08-24en
rioxxterms.versionofrecord10.3389/fmed.2017.00148en
rioxxterms.versionVoR*
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-08-24en
dc.contributor.orcidBradley, John [0000-0002-7774-8805]
dc.identifier.eissn2296-858X
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idAddenbrooke's Kidney Patients Association (AKPA/RL15)
pubs.funder-project-idAddenbrooke's Charitable Trust (ACT) (Minute No 14/16 C (ii))
pubs.funder-project-idCambridge University Hospitals NHS Foundation Trust (CUH) (BRC 2017-22)
cam.issuedOnline2017-09-11en


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International