© 2016 Elsevier Ltd. The concept of eco-industrial park (EIP) has recently become the subject of a great deal of attention from industry and academic research groups. This paper proposes a series of systematic approaches for multi-level modelling and optimisation in EIPs. The novelties of this work include, (1) building a four-level modelling framework (from unit level to process level, plant level and industrial network level) for EIP research, (2) applying advanced mathematical modelling methods to describe each level operation, (3) developing efficient methodologies for solving optimisation problems at different EIP levels, (4) considering symbiotic relations amongst the three networks (material, water and energy networks) at the top EIP level with the boundary conditions of economic, social and legal requirements. For methodology demonstration, two cases at process level and industrial network level respectively are tested and solved with the developed modelling and optimisation strategies. Finally, the challenges and applications in future EIP research are also discussed, including data collection, the extension of the current networks to EIPs, and the feasibility of the proposed methodologies for complex EIP problems. The extended EIPs include the combination of material exchanges, energy systems and waste-water treatment networks. The aspects considered for future industrial ecology are carbon emission, by-product reuse, water consumption, and energy consumption. The main object of this paper is to explain the detailed model construction process and the development of optimisation approaches for a complex EIP system. In future work, this system is expected to share services, utility, and product resources amongst industrial plants to add value, reduce costs, improve environment, and consequently achieve sustainable development in a symbiosis community.