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Escherichia coli tryptophanase (TnaA-GFP) expression at different stages of growth (flow cytometry)


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Description

We used flow cytometry to investigate intercellular distribution of E. coli tryptophanase, the enzyme that synthesize indole. The data fill the gap in studies of tryptophanase and corroborated previous works by other groups. We found that tryptophanase level in individual cells is highly heterogeneous.

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Software / Usage instructions

All original flow cytometry data are in fcs format; the zip files are named after the date of the experiment and there are 3 experiments in total. The sample optical density (OD) data is in csv format.

Keywords

Escherichia coli, tryptophanase, indole, TnaA-GFP, flow cytometry

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