Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts
Journal of Visualized Experiments
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Shrigley, S., Pircs, K., Barker, R., Parmar, M., & Drouin-Ouellet, J. (2018). Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts. Journal of Visualized Experiments, (132. e56904)https://doi.org/10.3791/56904
Direct neuronal reprogramming generates neurons to maintain the age of the starting somatic cell. Here, we describe a single vector-based method to generate induced neurons from dermal fibroblasts obtained from adult human donors.
Neuroscience, Issue 132, Induced neurons, adult human dermal fibroblasts, direct neural reprogramming, human neurons, FACS purification, lentiviral vector
We thank Marie Persson Vejgården for technical assistance. The research leading to these results has received funding from the New York Stem Cell Foundation, the European Research Council under the European Union’s Seventh Framework Programme: FP/2007-2013 Neuro Stem Cell Repair (no. 602278) and ERC Grant Agreement no. 30971, the Swedish Research Council (grant agreement 521-2012-5624, 2016-00873 and 70862601/ Bagadilico), Swedish Parkinson Foundation (Parkinsonfonden), and the Strategic Research Area at Lund University Multipark (multidisciplinary research in Parkinson’s disease). Janelle Drouin-Ouellet is supported by a Canadian Institutes of Health Research (CIHR) fellowship (#358492), and Roger Barker is supported by an NIHR Biomedical Research Centre grant to the University of Cambridge/Addenbrooke’s Hospital. Malin Parmar is a New York Stem Cell Foundation Robertson Investigator. Shelby Shrigley is funded by the European Union Horizon 2020 Programme (H2020-MSCA-ITN-2015) under the Marie Skłodowska-Curie Innovative Training Network and Grant Agreement No. 676408.
EC FP7 CP (602278)
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External DOI: https://doi.org/10.3791/56904
This record's URL: https://www.repository.cam.ac.uk/handle/1810/271233
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Licence URL: http://creativecommons.org/licenses/by-nc-nd/3.0/