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dc.contributor.authorLiu, W
dc.contributor.authorWilson, DI
dc.contributor.authorChen, XD
dc.contributor.authorMercadé-Prieto, R
dc.date.accessioned2018-01-30T17:35:47Z
dc.date.available2018-01-30T17:35:47Z
dc.date.issued2018
dc.identifier.issn1935-5130
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/271362
dc.description.abstractWide-field fluorescence microscopy was used to quantify the evolution of the volumetric swelling ratio, Q, i.e. solids content, in a protein hydrogel undergoing swelling and dissolution. Heat-induced whey protein hydrogels labelled with Rhodamine B isothiocyanate (RITC) were used as a model system. Complications in the quantification of Q using fluorescence of proteins conjugated RITC, arising from alkali destroying protein-dye interactions, were overcome using a reaction-diffusion numerical scheme. At pH 12-13, when the hydrogels dissolve readily, overlapping fluorescence intensity profiles were observed at different times, consistent with a system dissolving at steady state. In stronger alkali (e.g. 1 M NaOH), when dissolution proceeds very slowly, we confirm that there is little swelling next to the gel boundary. These results present the first quantification of the solids distribution within protein hydrogels under reactive conditions.
dc.publisherSpringer Science and Business Media LLC
dc.titleQuantification of the Local Protein Content in Hydrogels Undergoing Swelling and Dissolution at Alkaline pH Using Fluorescence Microscopy
dc.typeArticle
prism.endingPage584
prism.issueIdentifier3
prism.publicationDate2018
prism.publicationNameFood and Bioprocess Technology
prism.startingPage572
prism.volume11
dc.identifier.doi10.17863/CAM.18345
dc.identifier.doi10.17863/CAM.18345
dcterms.dateAccepted2017-11-12
rioxxterms.versionofrecord10.1007/s11947-017-2031-z
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-03-01
dc.contributor.orcidMercadé-Prieto, R [0000-0003-0487-7400]
dc.identifier.eissn1935-5149
rioxxterms.typeJournal Article/Review
cam.issuedOnline2017-11-25
rioxxterms.freetoread.startdate2018-11-25


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