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dc.contributor.authorRanasinghe, Rohan T
dc.contributor.authorChalland, Martin R
dc.contributor.authorGanzinger, Kristina A
dc.contributor.authorLewis, Benjamin W
dc.contributor.authorSoftley, Charlotte
dc.contributor.authorSchmied, Wolfgang H
dc.contributor.authorHorrocks, Mathew H
dc.contributor.authorShivji, Nadia
dc.contributor.authorChin, Jason W
dc.contributor.authorSpencer, James
dc.contributor.authorKlenerman, David
dc.date.accessioned2018-02-16T15:59:26Z
dc.date.available2018-02-16T15:59:26Z
dc.date.issued2018-02-13
dc.identifier.issn2041-1723
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/273301
dc.description.abstractMethylated bases in tRNA, rRNA and mRNA control a variety of cellular processes, including protein synthesis, antimicrobial resistance and gene expression. Currently, bulk methods that report the average methylation state of ~104-107 cells are used to detect these modifications, obscuring potentially important biological information. Here, we use in situ hybridization of Molecular Beacons for single-cell detection of three methylations (m62A, m1G and m3U) that destabilize Watson-Crick base pairs. Our method-methylation-sensitive RNA fluorescence in situ hybridization-detects single methylations of rRNA, quantifies antibiotic-resistant bacteria in mixtures of cells and simultaneously detects multiple methylations using multicolor fluorescence imaging.
dc.format.mediumElectronic
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rightsAttribution 4.0 International
dc.rightsAttribution 4.0 International
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectEscherichia coli
dc.subjectAdenine
dc.subjectGuanine
dc.subjectMethyltransferases
dc.subjectEscherichia coli Proteins
dc.subjectRNA
dc.subjectRNA, Ribosomal
dc.subjectUridine
dc.subjectMicroscopy, Fluorescence
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectMethylation
dc.subjectSingle-Cell Analysis
dc.titleDetecting RNA base methylations in single cells by in situ hybridization.
dc.typeArticle
prism.issueIdentifier1
prism.publicationDate2018
prism.publicationNameNat Commun
prism.startingPage655
prism.volume9
dc.identifier.doi10.17863/CAM.20234
dcterms.dateAccepted2017-12-20
rioxxterms.versionofrecord10.1038/s41467-017-02714-7
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.licenseref.startdate2018-02-13
dc.contributor.orcidRanasinghe, Rohan T [0000-0001-9227-4110]
dc.contributor.orcidChalland, Martin R [0000-0002-9685-3504]
dc.contributor.orcidGanzinger, Kristina A [0000-0001-9106-9406]
dc.contributor.orcidLewis, Benjamin W [0000-0003-3050-7577]
dc.contributor.orcidChin, Jason W [0000-0003-1219-4757]
dc.identifier.eissn2041-1723
rioxxterms.typeJournal Article/Review
pubs.funder-project-idEuropean Commission (216027)
pubs.funder-project-idEuropean Commission (115153)
cam.issuedOnline2018-02-13
cam.orpheus.successThu Jan 30 12:59:45 GMT 2020 - The item has an open VoR version.
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International