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dc.contributor.authorGiusto, Elenaen
dc.contributor.authorDonegà, Matteoen
dc.contributor.authorDumitru, Andra Cen
dc.contributor.authorFoschi, Giuliaen
dc.contributor.authorCasalini, Stefanoen
dc.contributor.authorBianchi, Micheleen
dc.contributor.authorLeonardi, Tommasoen
dc.contributor.authorRusso, Alessandroen
dc.contributor.authorOcchipinti, Luigien
dc.contributor.authorBiscarini, Fabioen
dc.contributor.authorGarcia, Ricardoen
dc.contributor.authorPluchino, Stefanoen
dc.date.accessioned2018-02-21T15:24:03Z
dc.date.available2018-02-21T15:24:03Z
dc.date.issued2017-04en
dc.identifier.issn2366-7478
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/273367
dc.description.abstractA quantitative method to assess the in vitro foreign body reaction (FBR) of mononuclear phagocytes (MP) to polymers relevant in implants for prosthetics, advanced therapies, and regenerative medicine is presented. It integrates single‐cell force spectroscopy (SCFS) with immunogenic profiles of the MPs. In cell force spectroscopy experiments a single phagocyte, linked at the end of an atomic force microscopy cantilever, probes the adhesion forces between the cell and the polymer surface. SCFS measures adhesion forces in a range from 10 pN to 100 nN and with spatial resolution from cell size down to nanometers, accessing the early adhesion events established at contact times between milliseconds and minutes. The time evolution within the first 60 s of the adhesion force between the phagocyte and the polymer surface before and after the treatment with an immunosuppressive drug, viz. Minocycline, a Federal Drug Administration (FDA)‐approved third generation tetracycline with anti‐inflammatory effects, is then studied. The adhesion force values measured at the single cell level is shown to correlate to the immunogenic profiles obtained by analysis of biomarkers and morphology of the MPs in culture. Also, Minocycline causes a decrease of both proinflammatory gene expression profiles and adhesive forces of single cells
dc.publisherWiley
dc.titleInterfacing Polymers and Tissues: Quantitative Local Assessment of the Foreign Body Reaction of Mononuclear Phagocytes to Polymeric Materialsen
dc.typeArticle
prism.issueIdentifier4en
prism.number1700021en
prism.publicationDate2017en
prism.publicationNameAdvanced Biosystemsen
prism.volume1en
dc.identifier.doi10.17863/CAM.20397
dcterms.dateAccepted2017-02-08en
rioxxterms.versionofrecord10.1002/adbi.201700021en
rioxxterms.versionAM*
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2017-04en
dc.contributor.orcidOcchipinti, Luigi [0000-0002-9067-2534]
dc.contributor.orcidPluchino, Stefano [0000-0002-6267-9472]
dc.identifier.eissn2366-7478
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEuropean Research Council (260511)
pubs.funder-project-idBascule Charitable Trust (RG75149)
pubs.funder-project-idGreat Britain Sasakawa Foundation (B89)
cam.issuedOnline2017-03-30en
rioxxterms.freetoread.startdate2018-03-30


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