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RNA-DamID reveals cell-type-specific binding of roX RNAs at chromatin-entry sites.

Accepted version
Peer-reviewed

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Type

Article

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Authors

Abstract

Thousands of long noncoding RNAs (lncRNAs) have been identified in eukaryotic genomes, many of which are expressed in spatially and temporally restricted patterns. Nonetheless, the roles of the majority of these transcripts are still unknown. One of the mechanisms by which lncRNAs function is through the modulation of chromatin states. To assess the functions of lncRNAs, we developed RNA-DamID, a novel approach that detects lncRNA-genome interactions in a cell-type-specific manner in vivo with high sensitivity and accuracy. Identifying the cell-type-specific genome occupancy of lncRNAs is vital to understanding their mechanisms of action in development and disease. We used RNA-DamID to investigate targeting of the lncRNAs in the Drosophila dosage-compensation complex (DCC) and show that initial targeting is cell-type specific.

Description

Keywords

Aminohydrolases, Animals, Chromatin, DNA-Binding Proteins, Dosage Compensation, Genetic, Drosophila Proteins, Drosophila melanogaster, Female, Genotype, Male, Neural Stem Cells, Nuclear Proteins, RNA, Long Noncoding, RNA-Binding Proteins, Salivary Glands, Transcription Factors

Journal Title

Nat Struct Mol Biol

Conference Name

Journal ISSN

1545-9993
1545-9985

Volume Title

25

Publisher

Springer Science and Business Media LLC
Sponsorship
Wellcome Trust (092545/Z/10/Z)
Wellcome Trust (103792/Z/14/Z)
Royal Society (RP150061)
Wellcome Trust (092096/Z/10/Z)
Cancer Research Uk (None)