RNA-DamID reveals cell-type-specific binding of roX RNAs at chromatin-entry sites.
Nature structural & molecular biology
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Cheetham, S. W., & Brand, A. (2018). RNA-DamID reveals cell-type-specific binding of roX RNAs at chromatin-entry sites.. Nature structural & molecular biology, 25 (1), 109-114. https://doi.org/10.1038/s41594-017-0006-4
Abstract Thousands of long noncoding RNAs (lncRNAs) have been identified in eukaryotic genomes, many of which are expressed in spatially and temporally restricted patterns. Nonetheless, the roles of the majority of these transcripts are still unknown. One of the mechanisms by which lncRNAs function is through the modulation of chromatin state. To assess the functions of lncRNAs we developed RNA-DamID, a novel approach that detects lncRNA-genome interactions in a cell-type specific manner in vivo with high sensitivity and accuracy. Identifying the cell-type-specific genome occupancy of lncRNAs is key to understanding their mechanisms of action in development and disease. We used RNA-DamID to investigate targeting of the lncRNAs in the Drosophila dosage compensation complex (DCC) and show that initial targeting is cell-type-specific.
Salivary Glands, Chromatin, Animals, Drosophila melanogaster, Aminohydrolases, RNA-Binding Proteins, DNA-Binding Proteins, Drosophila Proteins, Nuclear Proteins, Transcription Factors, Genotype, Dosage Compensation, Genetic, Female, Male, Neural Stem Cells, RNA, Long Noncoding
Wellcome Trust (092545/Z/10/Z)
WELLCOME TRUST (103792/Z/14/Z)
Royal Society (RP150061)
Wellcome Trust (092096/Z/10/Z)
Cancer Research UK (A14492)
External DOI: https://doi.org/10.1038/s41594-017-0006-4
This record's URL: https://www.repository.cam.ac.uk/handle/1810/273463