Engineering the Drosophila Genome for Developmental Biology.
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Peer-reviewed
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Abstract
The recent development of transposon and CRISPR-Cas9-based tools for manipulating the fly genome in vivo promises tremendous progress in our ability to study developmental processes. Tools for introducing tags into genes at their endogenous genomic loci facilitate imaging or biochemistry approaches at the cellular or subcellular levels. Similarly, the ability to make specific alterations to the genome sequence allows much more precise genetic control to address questions of gene function.
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Keywords
CRISPR-Cas9, Drosophila, genome engineering, protein tagging
Journal Title
J Dev Biol
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Journal ISSN
2221-3759
2221-3759
2221-3759
Volume Title
5
Publisher
MDPI AG
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Sponsorship
Biotechnology and Biological Sciences Research Council (BB/N007069/1)
BBSRC (via University of Manchester) (R116574-LLM)
Biotechnology and Biological Sciences Research Council (BB/L002817/1)
BBSRC (via University of Manchester) (R116574-LLM)
Biotechnology and Biological Sciences Research Council (BB/L002817/1)
BBSRC BB/L002817/1 and BB/N007069/1