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Extracellular Lactate: A Novel Measure of T Cell Proliferation.

Published version
Peer-reviewed

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Authors

Georgieva, Zoya 
Thompson, Sara 
Kaur Sandhu, Harpreet 

Abstract

Following activation, T cells rapidly divide and acquire effector functions. This energetically demanding process depends upon the ability of T cells to undergo metabolic remodeling from oxidative phosphorylation to aerobic glycolysis, during which glucose is converted into lactate and released extracellularly. In this article, we demonstrate that extracellular lactate can be used to dynamically assess human T cell responses in vitro. Extracellular lactate levels strongly correlated with T cell proliferation, and measuring lactate compared favorably with traditional methods for determining T cell responses (i.e., [3H]thymidine incorporation and the use of cell proliferation dyes). Furthermore, we demonstrate the usefulness of measuring lactate as a read-out in conventional suppression assays and high-throughput peptide-screening assays. Extracellular lactate was stably produced over 7 d, and results were reproducibly performed over several freeze-thaw cycles. We conclude that the use of extracellular lactate measurements can be a sensitive, safe, stable, and easy-to-implement research tool for measuring T cell responses and cellular metabolic changes in vitro.

Description

Keywords

CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Cell Proliferation, Cells, Cultured, Cytomegalovirus, Glycolysis, Humans, Lactic Acid, Lymphocyte Activation, Oxidative Phosphorylation, Viral Proteins

Journal Title

J Immunol

Conference Name

Journal ISSN

0022-1767
1550-6606

Volume Title

200

Publisher

The American Association of Immunologists
Sponsorship
Wellcome Trust (105924/Z/14/Z)
Wellcome Trust (105924/Z/14/A)
Wellcome Trust (105924/Z/14/A)
MRC (1508766)
Medical Research Council (G0701279)
Medical Research Council (G9202171)
Medical Research Council (MR/K021087/1)