Rapid, selective and stable HaloTag-Lb ADH immobilization directly from crude cell extract for the continuous biocatalytic production of chiral alcohols and epoxides
Reaction Chemistry and Engineering
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Döbber, J., Pohl, M., Ley, S., & Musio, B. (2018). Rapid, selective and stable HaloTag-Lb ADH immobilization directly from crude cell extract for the continuous biocatalytic production of chiral alcohols and epoxides. Reaction Chemistry and Engineering, 3 (1), 8-12. https://doi.org/10.1039/c7re00173h
A strategy for biocatalyst immobilization in flow directly from the crude cell extract is described. The efficiency and the stability of the immobilized enzyme were demonstrated during the asymmetric reduction of a range of ketones. The cascade two-step chemo-enzymatic preparation of chiral epoxides was possible through the initial ketone bioreduction to an intermediate halohydrin followed by its intramolecular cyclization.
EPSRC (Award Nos. EP/K009494/1 and EP/K039520/1), the German Federal Ministry of Education and Research (BMBF) within the project “Molecular Interaction Engineering” (funding code 031A095).
External DOI: https://doi.org/10.1039/c7re00173h
This record's URL: https://www.repository.cam.ac.uk/handle/1810/273522
Attribution 4.0 International
Licence URL: http://creativecommons.org/licenses/by/4.0/
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