Homographically generated light sheets for the microscopy of large specimens.
Published version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Russell, Craig T
Rees, Eric J
Kaminski, Clemens F
Abstract
We compare the performance of linear and nonlinear methods for aligning the excitation and detection planes throughout volumes of large specimens in digitally scanned light sheet microscopy. An effective nonlinear method involves the registration of four corner extrema of the imaging volume via a projective transform. We show that this improves the light collection efficiency of the commonly used three-point affine registration by an average of 42% over a typical specimen volume. Accurate illumination/detection registration methods are now pertinent to biological research in view of current trends towards imaging large or expanded samples, at depth, with diffraction limited resolution.
Description
Keywords
Fluorescent Dyes, Imaging, Three-Dimensional, Light, Microscopy
Journal Title
Opt Lett
Conference Name
Journal ISSN
0146-9592
1539-4794
1539-4794
Volume Title
43
Publisher
The Optical Society
Publisher DOI
Sponsorship
EPSRC (1362132)
Engineering and Physical Sciences Research Council (EP/H018301/1)
Medical Research Council (MR/K02292X/1)
Medical Research Council (MR/K015850/1)
Wellcome Trust (089703/Z/09/Z)
Biotechnology and Biological Sciences Research Council (BB/H023917/1)
Medical Research Council (G0902243)
Engineering and Physical Sciences Research Council (EP/L015889/1)
Engineering and Physical Sciences Research Council (EP/H018301/1)
Medical Research Council (MR/K02292X/1)
Medical Research Council (MR/K015850/1)
Wellcome Trust (089703/Z/09/Z)
Biotechnology and Biological Sciences Research Council (BB/H023917/1)
Medical Research Council (G0902243)
Engineering and Physical Sciences Research Council (EP/L015889/1)