Homographically generated light sheets for the microscopy of large specimens.
Publication Date
2018-02Journal Title
Optics letters
ISSN
0146-9592
Volume
43
Issue
4
Pages
663-666
Language
eng
Type
Article
This Version
VoR
Physical Medium
Print
Metadata
Show full item recordCitation
Russell, C., Rees, E., & Kaminski, C. (2018). Homographically generated light sheets for the microscopy of large specimens.. Optics letters, 43 (4), 663-666. https://doi.org/10.1364/ol.43.000663
Abstract
We compare the performance of linear and nonlinear methods for aligning the excitation and detection planes throughout volumes of large specimens in digitally scanned light sheet microscopy. An effective nonlinear method involves the registration of four corner extrema of the imaging volume via a projective transform. We show that this improves on the light collection efficiency of the commonly used 3-point affine registration by an average of 42% over a typical specimen volume. Accurate illumination/detection registration methods are now pertinent to biological research in view of current trends towards imaging large or expanded samples, at depth, with diffraction limited resolution.
Keywords
Fluorescent Dyes, Imaging, Three-Dimensional, Microscopy, Light
Sponsorship
EPSRC (1362132)
EPSRC (EP/H018301/1)
MRC (MR/K02292X/1)
MRC (MR/K015850/1)
Wellcome Trust (089703/Z/09/Z)
BBSRC (BB/H023917/1)
MRC (G0902243)
EPSRC (EP/L015889/1)
Embargo Lift Date
2100-01-01
Identifiers
External DOI: https://doi.org/10.1364/ol.43.000663
This record's URL: https://www.repository.cam.ac.uk/handle/1810/273591
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