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dc.contributor.authorPage, Mike Ten
dc.contributor.authorMcCormac, Alex Cen
dc.contributor.authorSmith, Alisonen
dc.contributor.authorTerry, Matthew Jen
dc.date.accessioned2018-02-28T18:33:11Z
dc.date.available2018-02-28T18:33:11Z
dc.date.issued2017-02en
dc.identifier.issn0028-646X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/273643
dc.description.abstractRetrograde signals from the plastid regulate photosynthesis-associated nuclear genes and are essential to successful chloroplast biogenesis. One model is that a positive haem-related signal promotes photosynthetic gene expression in a pathway that is abolished by the herbicide norflurazon. Far-red light (FR) pretreatment and transfer to white light also results in plastid damage and loss of photosynthetic gene expression. Here, we investigated whether norflurazon and FR pretreatment affect the same retrograde signal. We used transcriptome analysis and real-time reverse transcription-polymerase chain reaction (RT-PCR) to analyse the effects of these treatments on nuclear gene expression in various Arabidopsis (Arabidopsis thaliana) retrograde signalling mutants. Results showed that the two treatments inhibited largely different nuclear gene sets, suggesting that they affected different retrograde signals. Moreover, FR pretreatment resulted in singlet oxygen ((1) O2 ) production and a rapid inhibition of photosynthetic gene expression. This inhibition was partially blocked in the executer1executer2 mutant, which is impaired in (1) O2 signalling. Our data support a new model in which a (1) O2 retrograde signal, generated by chlorophyll precursors, inhibits expression of key photosynthetic and chlorophyll synthesis genes to prevent photo-oxidative damage during de-etiolation. Such a signal would provide a counterbalance to the positive haem-related signal to fine tune regulation of chloroplast biogenesis.
dc.description.sponsorshipThis work was funded by BBSRC grants 51/P17214 and BB/ J018139/1 to M.J.T. and BB/J018694/1 to A.G.S.
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherWiley-Blackwell
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectPlastidsen
dc.subjectArabidopsisen
dc.subjectSinglet Oxygenen
dc.subjectTetrapyrrolesen
dc.subjectSignal Transductionen
dc.subjectPhotosynthesisen
dc.subjectTranscription, Geneticen
dc.subjectGene Expression Regulation, Planten
dc.subjectMutationen
dc.subjectLighten
dc.subjectModels, Biologicalen
dc.subjectTime Factorsen
dc.titleSinglet oxygen initiates a plastid signal controlling photosynthetic gene expression.en
dc.typeArticle
prism.endingPage1180
prism.issueIdentifier3en
prism.publicationDate2017en
prism.publicationNameThe New phytologisten
prism.startingPage1168
prism.volume213en
dc.identifier.doi10.17863/CAM.20704
dcterms.dateAccepted2016-08-19en
rioxxterms.versionofrecord10.1111/nph.14223en
rioxxterms.versionVoR*
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-02en
dc.contributor.orcidPage, Mike T [0000-0002-9715-7076]
dc.contributor.orcidSmith, Alison [0000-0001-6511-5704]
dc.contributor.orcidTerry, Matthew J [0000-0001-5002-2708]
dc.identifier.eissn1469-8137
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/J018694/1)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International