Defining the earliest step of cardiovascular lineage segregation by single-cell RNA-seq.
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Authors
Gargouri, Souhir
Dubois, Christine
Paulissen, Catherine
Gottgens, Berthold
Publication Date
2018-03Journal Title
Science (New York, N.Y.)
ISSN
0036-8075
Volume
359
Issue
6380
Pages
1177-1181
Language
eng
Type
Article
This Version
AM
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Lescroart, F., Wang, X., Lin, X., Swedlund, B., Gargouri, S., Sànchez-Dànes, A., Moignard, V., et al. (2018). Defining the earliest step of cardiovascular lineage segregation by single-cell RNA-seq.. Science (New York, N.Y.), 359 (6380), 1177-1181. https://doi.org/10.1126/science.aao4174
Abstract
Mouse heart development arises from Mesp1 expressing cardiovascular progenitors (CPs) that are specified during gastrulation. The molecular processes that control early regional and lineage segregation of CPs remain unclear. Here, we performed single cell RNA-sequencing of WT and Mesp1 null CPs. We showed that Mesp1 CPs are molecularly distinct and make the continuum between epiblast and later mesodermal cells including hematopoietic progenitors. Single cell transcriptome analysis of Mesp1 deficient CPs showed that Mesp1 is required for the exit from the pluripotent state and the induction of the cardiovascular gene expression program. We identified distinct populations of Mesp1 CPs that correspond to progenitors committed to different cell lineages and regions of the heart, identifying the molecular features associated with early lineage restriction and regional segregation of the heart at the early stage of gastrulation.
Keywords
Heart, Hematopoietic Stem Cells, Stem Cells, Mesoderm, Animals, Mice, Mice, Mutant Strains, RNA, Sequence Analysis, RNA, Cell Division, Gene Expression Regulation, Developmental, Cell Lineage, Basic Helix-Loop-Helix Transcription Factors
Sponsorship
Leukaemia & Lymphoma Research (12029)
WELLCOME TRUST (105031/D/14/Z)
Cancer Research UK (21762)
National Institutes of Health (NIH) (via Pennsylvania State University) (R24DK106766)
Wellcome Trust (206328/Z/17/Z)
MEDICAL RESEARCH COUNCIL (MR/M008975/1)
MRC (MC_PC_12009)
Identifiers
External DOI: https://doi.org/10.1126/science.aao4174
This record's URL: https://www.repository.cam.ac.uk/handle/1810/274106
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