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dc.contributor.authorGoussous, Samien
dc.contributor.authorCasford, Michaelen
dc.contributor.authorMurphy, ACen
dc.contributor.authorSalmond, Georgeen
dc.contributor.authorLeeper, Finianen
dc.contributor.authorDavies, Paulen
dc.date.accessioned2018-03-26T07:48:23Z
dc.date.available2018-03-26T07:48:23Z
dc.date.issued2017-05-18en
dc.identifier.issn1520-6106
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/274313
dc.description.abstractThe lipopeptide surfactin produced by certain strains of Bacillus subtilis is a powerful biosurfactant possessing potentially useful antimicrobial properties. In order to better understand its surface behavior, we have used surface sensitive sum frequency generation (SFG) vibrational spectroscopy in the C-H and C═O stretching regions to determine its structure at the air/water interface. Using surfactin with the leucine groups of the peptide ring perdeuterated, we have shown that a majority of the SFG signals arise from the 4 leucine residues. We find that surfactin forms a robust film, and that its structure is not affected by the number density at the interface or by pH variation of the subphase. The spectra show that the ring of the molecule lies in the plane of the surface rather than perpendicular to it, with the tail lying above this, also in the plane of the interface.
dc.description.sponsorshipWe thank Unilever R&D and the EPSRC(UK) for an iCASE studentship for S.A.G. on Grant 1505859, and the BBSRC(UK) for a postdoctoral research assistantship for A.C.M. on Grant BB/N008081/1 to G.P.C.S. and F.J.L.
dc.languageengen
dc.publisherAmerican Chemical Society
dc.titleStructure of the Fundamental Lipopeptide Surfactin at the Air/Water Interface Investigated by Sum Frequency Generation Spectroscopyen
dc.typeArticle
prism.endingPage5077
prism.issueIdentifier19en
prism.publicationDate2017en
prism.publicationNameJournal of Physical Chemistry Ben
prism.startingPage5072
prism.volume121en
dc.identifier.doi10.17863/CAM.21436
dcterms.dateAccepted2017-04-24en
rioxxterms.versionofrecord10.1021/acs.jpcb.7b03476en
rioxxterms.versionAM*
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2017-05-18en
dc.contributor.orcidCasford, Michael [0000-0003-1373-5383]
dc.contributor.orcidSalmond, George [0000-0002-5197-2198]
dc.contributor.orcidLeeper, Finian [0000-0003-3408-5199]
dc.contributor.orcidDavies, Paul [0000-0001-7314-2243]
dc.identifier.eissn1520-5207
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/N008081/1)
pubs.funder-project-idEPSRC (1505859)
pubs.funder-project-idBBSRC (BB/K001833/1)
cam.issuedOnline2017-04-24en
rioxxterms.freetoread.startdate2018-04-24


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