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dc.contributor.authorDavis, FMen
dc.contributor.authorLloyd-Lewis, Bethanen
dc.contributor.authorHarris, OBen
dc.contributor.authorKozar, Sen
dc.contributor.authorWinton, Douglasen
dc.contributor.authorMuresan, Leilaen
dc.contributor.authorWatson, Christineen
dc.date.accessioned2018-04-12T17:12:36Z
dc.date.available2018-04-12T17:12:36Z
dc.date.issued2016-10-25en
dc.identifier.issn2041-1723
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/274820
dc.description.abstractThe mammary gland undergoes cycles of growth and regeneration throughout reproductive life, a process that requires mammary stem cells (MaSCs). Whilst recent genetic fate-mapping studies using lineage-specific promoters have provided valuable insights into the mammary epithelial hierarchy, the true differentiation potential of adult MaSCs remains unclear. To address this, herein we utilize a stochastic genetic-labelling strategy to indelibly mark a single cell and its progeny in situ, combined with tissue clearing and 3D imaging. Using this approach, clones arising from a single parent cell could be visualized in their entirety. We reveal that clonal progeny contribute exclusively to either luminal or basal lineages and are distributed sporadically to branching ducts or alveoli. Quantitative analyses suggest that pools of unipotent stem/progenitor cells contribute to adult mammary gland development. Our results highlight the utility of tracing a single cell and reveal that progeny of a single proliferative MaSC/progenitor are dispersed throughout the epithelium.
dc.description.sponsorshipThis work was supported by a grant from the Medical Research Council programme grant no. MR/J001023/1 (B.L-L. and C.J.W). F.M.D. was funded by a National Health and Medical Research Council CJ Martin Biomedical Fellowship (GNT1071074). O.B.H. was funded by a Wellcome Trust PhD studentship (105377/Z/14/Z).
dc.languageENGen
dc.publisherSpringer Nature
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleSingle-cell lineage tracing in the mammary gland reveals stochastic clonal dispersion of stem/progenitor cell progeny.en
dc.typeArticle
prism.endingPage13053
prism.publicationDate2016en
prism.publicationNameNature Communicationsen
prism.startingPage13053
prism.volume7en
dc.identifier.doi10.17863/CAM.21969
dcterms.dateAccepted2016-08-30en
rioxxterms.versionofrecord10.1038/ncomms13053en
rioxxterms.versionVoR*
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2016-10-25en
dc.contributor.orcidWinton, Douglas [0000-0001-6067-7927]
dc.contributor.orcidMuresan, Leila [0000-0002-7602-0249]
dc.contributor.orcidWatson, Christine [0000-0002-8548-5902]
dc.identifier.eissn2041-1723
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idCancer Research UK (C14303_do not transfer)
pubs.funder-project-idMRC (MR/J001023/1)
pubs.funder-project-idMRC (MC_PC_12009)
pubs.funder-project-idWELLCOME TRUST (103805/Z/14/Z)
pubs.funder-project-idCancer Research UK (CB4230)
pubs.funder-project-idWELLCOME TRUST (105377/Z/14/Z)
cam.issuedOnline2016-10-25en


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International