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dc.contributor.authorGoodband, Emily Len
dc.contributor.authorSerrano, Gonçaloen
dc.contributor.authorConstantino-Casas, Fernandoen
dc.contributor.authorArcher, Joyen
dc.contributor.authorWatson, Penelopeen
dc.contributor.authorWilliams, Timothyen
dc.date.accessioned2018-04-19T10:50:20Z
dc.date.available2018-04-19T10:50:20Z
dc.date.issued2018-01en
dc.identifier.issn2052-6113
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/275031
dc.description.abstractThe objectives of this study were fourfold: technical validation of a commercial canine 1,2-o-dilauryl-rac-glycero glutaric acid-(6'-methylresorufin) ester (DGGR) lipase assay, to calculate a reference interval for DGGR lipase by the indirect a posteriori method, to establish biological validity of the assay, and to assess agreement between DGGR lipase and specific canine pancreatic lipase (Spec cPL) assays. Dogs with histologically confirmed acute pancreatitis (n=3), chronic pancreatitis (n=8) and normal pancreatic tissue (n=7) with stored (-80°C) serum samples were identified. Relevant controls were selected. Precision, reproducibility and linearity of DGGR lipase, and the effect of sample haemolysis and freezing, were assessed. Sensitivity and specificity of DGGR lipase and Spec cPL were determined. Agreement between these two parameters was calculated using Cohen's kappa coefficient (κ). The DGGR lipase assay demonstrated excellent precision, reproducibility and linearity. Sample haemolysis and storage at -80°C for 12 months did not influence the assay. DGGR lipase (>245IU/l) and Spec cPL (>400µg/l) both showed poor sensitivity but excellent specificity for acute pancreatitis, and poor to moderate sensitivity but excellent specificity for chronic pancreatitis. Substantial agreement (κ=0.679) was found between DGGR lipase and Spec cPL. The validated DGGR lipase assay had similar sensitivity and specificity for the diagnosis of acute and chronic pancreatitis to Spec cPL. DGGR lipase is a reliable alternative to Spec cPL for the diagnosis of pancreatitis.
dc.format.mediumElectronic-eCollectionen
dc.languageengen
dc.publisherBMJ
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleValidation of a commercial 1,2-o-dilauryl-rac-glycero glutaric acid-(6'-methylresorufin) ester lipase assay for diagnosis of canine pancreatitis.en
dc.typeArticle
prism.issueIdentifier1en
prism.publicationDate2018en
prism.publicationNameVeterinary record openen
prism.startingPagee000270
prism.volume5en
dc.identifier.doi10.17863/CAM.22198
dcterms.dateAccepted2018-04-11en
rioxxterms.versionofrecord10.1136/vetreco-2017-000270en
rioxxterms.versionVoR*
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-01en
dc.contributor.orcidWatson, Penelope [0000-0002-7241-9412]
dc.contributor.orcidWilliams, Timothy [0000-0002-9775-8285]
dc.identifier.eissn2052-6113
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idPetSavers (AA0614)
cam.orpheus.successThu Jan 30 13:00:36 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International