Optical Voltage Sensing Using DNA Origami.
Hemmig, Elisa A
Ochmann, Sarah E
American Chemical Society
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Hemmig, E. A., Fitzgerald, C., Maffeo, C., Hecker, L., Ochmann, S. E., Aksimentiev, A., Tinnefeld, P., & et al. (2018). Optical Voltage Sensing Using DNA Origami.. Nano letters, 18 (3), 1962-1971. https://doi.org/10.1021/acs.nanolett.7b05354
We explore the potential of DNA nanotechnology for developing novel optical voltage sensing nano-devices that convert a local change of electric potential into optical signals. As a proof-of-concept of the sensing mechanism, we assembled voltage responsive DNA origami structures labelled with a single pair of FRET dyes. The DNA structures were reversibly immobilised on a nanocapillary tip and underwent controlled structural changes upon application of an electric field. The applied field was monitored through a change in FRET efficiency. By exchanging the position of a single dye, we could tune the voltage sensitivity of our DNA origami structure, demonstrating the flexibility and versatility of our approach. The experimental studies were complemented by coarse-grained simulations that characterised voltage-dependent elastic deformation of the DNA nanostructures and the associated change in the distance between the FRET pair. Our work opens a novel pathway for determining the mechanical properties of DNA origami structures, and highlights potential applications of dynamic DNA nanostructures as voltage sensors.
DNA, Fluorescent Dyes, Fluorescence Resonance Energy Transfer, Equipment Design, Biosensing Techniques, Electrodes, Electricity, Ion Transport, Nucleic Acid Conformation, Nanotechnology, Nanostructures
ECH2020 EUROPEAN RESEARCH COUNCIL (ERC) (647144)
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External DOI: https://doi.org/10.1021/acs.nanolett.7b05354
This record's URL: https://www.repository.cam.ac.uk/handle/1810/275373
Attribution 4.0 International
Licence URL: http://creativecommons.org/licenses/by/4.0/
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