Cross-reactivity of anti-HMGB1 antibodies for HMGB2.
Davies, Jessica E
Apta, Bonita HR
Journal of immunological methods
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Davies, J. E., Apta, B. H., & Harper, M. T. (2018). Cross-reactivity of anti-HMGB1 antibodies for HMGB2.. Journal of immunological methods, 456 72-76. https://doi.org/10.1016/j.jim.2018.02.006
HMGB1 and HMGB2 are DNA-interacting proteins but can also have extracellular actions during inflammation. Despite their relatively high homology, they may have distinct roles, making it essential to be able to differentiate between the two. Here we examine the specificity of five commercially-available anti-HMGB1 antibodies. By western blotting of recombinant proteins and HMGB1-/- mouse embryonic fibroblasts, we identified only one HMGB1 antibody that, under our experimental conditions, did not also detect HMGB2. Selecting specific antibodies for HMGB1 and HMGB2 allowed identification of distinct HMGB1 and HMGB2 subcellular pools in primary neutrophils.
British Heart Foundation (PG/16/45/32152)
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External DOI: https://doi.org/10.1016/j.jim.2018.02.006
This record's URL: https://www.repository.cam.ac.uk/handle/1810/275439
Attribution 4.0 International
Licence URL: http://creativecommons.org/licenses/by/4.0/
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