Localised dynactin protects growing microtubules to deliver oskar mRNA to the posterior cortex of the Drosophila oocyte.
Authors
Nieuwburg, Ross
Khuc Trong, Philipp
Publication Date
2017-10-16Journal Title
eLife
ISSN
2050-084X
Volume
6
Language
eng
Type
Article
This Version
VoR
Later Version(s)
Physical Medium
Electronic
Metadata
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Nieuwburg, R., Nashchekin, D., Jakobs, M., Carter, A. P., Khuc Trong, P., Goldstein, R., & St Johnston, R. (2017). Localised dynactin protects growing microtubules to deliver oskar mRNA to the posterior cortex of the Drosophila oocyte.. eLife, 6 https://doi.org/10.7554/eLife.27237
Abstract
© Nieuwburg et al. The localisation of oskar mRNA to the posterior of the Drosophila oocyte defines where the abdomen and germ cells form in the embryo. Kinesin 1 transports oskar mRNA to the oocyte posterior along a polarised microtubule cytoskeleton that grows from non-centrosomal microtubule organising centres (ncMTOCs) along the anterior/lateral cortex. Here, we show that the formation of this polarised microtubule network also requires the posterior regulation of microtubule growth. A missense mutation in the dynactin Arp1 subunit causes most oskar mRNA to localise in the posterior cytoplasm rather than cortically. oskar mRNA transport and anchoring are normal in this mutant, but the microtubules fail to reach the posterior pole. Thus, dynactin acts as an anti-catastrophe factor that extends microtubule growth posteriorly. Kinesin 1 transports dynactin to the oocyte posterior, creating a positive feedback loop that increases the length and persistence of the posterior microtubules that deliver oskar mRNA to the cortex.
Keywords
Oocytes, Microtubules, Animals, Drosophila melanogaster, Kinesin, Cytoskeletal Proteins, Drosophila Proteins, RNA, Messenger
Sponsorship
Wellcome Trust (080007/B/06/Z)
Cancer Research Uk (None)
Embargo Lift Date
2100-01-01
Identifiers
External DOI: https://doi.org/10.7554/eLife.27237
This record's URL: https://www.repository.cam.ac.uk/handle/1810/275505
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