On-chip measurements of protein unfolding from direct observations of micron-scale diffusion.
Authors
Yates, Emma V
Hong, Liu
Dobson, Christopher
Publication Date
2018-04Journal Title
Chemical science
ISSN
2041-6520
Publisher
Chemical Science
Volume
9
Issue
14
Pages
3503-3507
Language
eng
Type
Article
This Version
VoR
Physical Medium
Electronic-eCollection
Metadata
Show full item recordCitation
Zhang, Y., Yates, E. V., Hong, L., Saar, K., Meisl, G., Dobson, C., Knowles, T. P., & et al. (2018). On-chip measurements of protein unfolding from direct observations of micron-scale diffusion.. Chemical science, 9 (14), 3503-3507. https://doi.org/10.1039/c7sc04331g
Abstract
Investigations of protein folding, unfolding and stability are critical for the understanding of the molecular basis of biological structure and function. We describe here a microfluidic approach to probe the unfolding of unlabelled protein molecules in microliter volumes. We achieve this objective through the use of a microfluidic platform, which allows the changes in molecular diffusivity upon folding and unfolding to be detected directly. We illustrate this approach by monitoring the unfolding of bovine serum albumin in solution as a function of pH. These results show the viability of probing protein stability on chip in small volumes.
Sponsorship
BBSRC (BB/J002119/1)
European Research Council (337969)
Embargo Lift Date
2100-01-01
Identifiers
External DOI: https://doi.org/10.1039/c7sc04331g
This record's URL: https://www.repository.cam.ac.uk/handle/1810/275846
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