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Interactions between N-linked glycosylation and polymerisation of neuroserpin within the endoplasmic reticulum.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Moriconi, Claudia 
Ordoñez, Adriana 
Lupo, Giuseppe 
Gooptu, Bibek 
Irving, James A 

Abstract

The neuronal serpin neuroserpin undergoes polymerisation as a consequence of point mutations that alter its conformational stability, leading to a neurodegenerative dementia called familial encephalopathy with neuroserpin inclusion bodies (FENIB). Neuroserpin is a glycoprotein with predicted glycosylation sites at asparagines 157, 321 and 401. We used site-directed mutagenesis, transient transfection, western blot, metabolic labelling and ELISA to probe the relationship between glycosylation, folding, polymerisation and degradation of neuroserpin in validated cell models of health and disease. Our data show that glycosylation at N157 and N321 plays an important role in maintaining the monomeric state of neuroserpin, and we propose this is the result of steric hindrance or effects on local conformational dynamics that can contribute to polymerisation. Asparagine residue 401 is not glycosylated in wild type neuroserpin and in several polymerogenic variants that cause FENIB, but partial glycosylation was observed in the G392E mutant of neuroserpin that causes severe, early-onset dementia. Our findings indicate that N401 glycosylation reports lability of the C-terminal end of neuroserpin in its native state. This C-terminal lability is not required for neuroserpin polymerisation in the endoplasmic reticulum, but the additional glycan facilitates degradation of the mutant protein during proteasomal impairment. In summary, our results indicate how normal and variant-specific N-linked glycosylation events relate to intracellular folding, misfolding, degradation and polymerisation of neuroserpin.

Description

Keywords

conformational disease, neurodegeneration, protein aggregation, serpin, serpinopathies, Amino Acid Sequence, Animals, Biopolymers, COS Cells, Cells, Cultured, Chlorocebus aethiops, Endoplasmic Reticulum, Glycosylation, Humans, Models, Molecular, Mutation, Neuropeptides, PC12 Cells, Polymerization, Rats, Serpins, Neuroserpin

Journal Title

FEBS J

Conference Name

Journal ISSN

1742-464X
1742-4658

Volume Title

282

Publisher

Wiley
Sponsorship
Medical Research Council (G0601840)
Medical Research Council (G0901786)
Medical Research Council (G1002610)
Alpha One Foundation (unknown)
Wellcome Trust (100140/Z/12/Z)