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dc.contributor.authorMauri, Emanueleen
dc.contributor.authorSacchetti, Alessandroen
dc.contributor.authorVicario, Nunzioen
dc.contributor.authorPeruzzotti Jametti, Lucaen
dc.contributor.authorRossi, Filippoen
dc.contributor.authorPluchino, Stefanoen
dc.date.accessioned2018-05-21T10:34:27Z
dc.date.available2018-05-21T10:34:27Z
dc.date.issued2018-02en
dc.identifier.issn2047-4830
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/275998
dc.description.abstractThe use of neural stem cells (NSCs) in cell therapy has become a powerful tool used for the treatment of central nervous system diseases, including traumatic brain and spinal cord injuries. However, a significant drawback is related to the limited viability after transplantation in situ. The design of three-dimensional (3D) scaffolds that are capable of resembling the architecture and physico-chemical features of an extracellular environment could be a suitable approach to improve cell survival and preserve their cellular active phase over time. In this study, we investigated NSC adhesion and proliferation in hydrogel systems. In particular, we evaluated the effect of RGD binding domains on cell fate within the polymeric scaffold. The introduction of a tripeptide via hydrogel chemical functionalization improved the percentage of proliferating cells until 8 days after seeding when compared to the unmodified scaffold. The beneficial effects of this 3D culture system was further evident when compared to a NSC monolayer (2D) culture, resulting in an approximately 40% increase in cells in the active phases at 4 and 8 days, and maintained a difference of 25% until 21 days after seeding.
dc.format.mediumPrinten
dc.languageengen
dc.publisherRoyal Society of Chemistry (RSC)
dc.subjectAnimalsen
dc.subjectMice, Inbred C57BLen
dc.subjectMiceen
dc.subjectOligopeptidesen
dc.subjectHydrogelsen
dc.subjectTissue Engineeringen
dc.subjectCell Adhesionen
dc.subjectCell Proliferationen
dc.subjectProtein Bindingen
dc.subjectFemaleen
dc.subjectTissue Scaffoldsen
dc.subjectNeural Stem Cellsen
dc.titleEvaluation of RGD functionalization in hybrid hydrogels as 3D neural stem cell culture systems.en
dc.typeArticle
prism.endingPage510
prism.issueIdentifier3en
prism.publicationDate2018en
prism.publicationNameBiomaterials scienceen
prism.startingPage501
prism.volume6en
dc.identifier.doi10.17863/CAM.23279
dcterms.dateAccepted2018-01-08en
rioxxterms.versionofrecord10.1039/c7bm01056gen
rioxxterms.versionAM*
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-02en
dc.contributor.orcidMauri, Emanuele [0000-0002-6203-8405]
dc.contributor.orcidSacchetti, Alessandro [0000-0002-4830-0825]
dc.contributor.orcidVicario, Nunzio [0000-0001-5934-3962]
dc.contributor.orcidPeruzzotti-Jametti, Luca [0000-0002-9396-5607]
dc.contributor.orcidRossi, Filippo [0000-0003-2665-120X]
dc.contributor.orcidPluchino, Stefano [0000-0002-6267-9472]
dc.identifier.eissn2047-4849
rioxxterms.typeJournal Article/Reviewen
rioxxterms.freetoread.startdate2019-01-09


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