An Adaptable Phospholipid Membrane Mimetic System for Solution NMR Studies of Membrane Proteins.
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Peer-reviewed
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Abstract
Based on the saposin-A (SapA) scaffold protein, we demonstrate the suitability of a size-adaptable phospholipid membrane-mimetic system for solution NMR studies of membrane proteins (MPs) under close-to-native conditions. The Salipro nanoparticle size can be tuned over a wide pH range by adjusting the saposin-to-lipid stoichiometry, enabling maintenance of sufficiently high amounts of phospholipid in the Salipro nanoparticle to mimic a realistic membrane environment while controlling the overall size to enable solution NMR for a range of MPs. Three representative MPs, including one G-protein-coupled receptor, were successfully incorporated into SapA-dimyristoylphosphatidylcholine nanoparticles and studied by solution NMR spectroscopy.
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Keywords
Biomimetics, Dimyristoylphosphatidylcholine, Hydrogen-Ion Concentration, Magnetic Resonance Spectroscopy, Membrane Proteins, Membranes, Artificial, Nanoparticles, Phospholipids, Receptors, G-Protein-Coupled, Saposins
Journal Title
J Am Chem Soc
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Journal ISSN
0002-7863
1520-5126
1520-5126
Volume Title
139
Publisher
American Chemical Society (ACS)
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Sponsorship
Biotechnology and Biological Sciences Research Council (BB/K01983X/1)
MRC (MR/L014254/1)
MRC (MR/L014254/1)