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dc.contributor.authorChien, Chih-Ta Henryen
dc.contributor.authorHelfinger, Lukas Ren
dc.contributor.authorBostock, Marken
dc.contributor.authorSolt, Andrasen
dc.contributor.authorTan, Yi Leien
dc.contributor.authorNietlispach, Danielen
dc.date.accessioned2018-05-31T11:00:20Z
dc.date.available2018-05-31T11:00:20Z
dc.date.issued2017-10-10en
dc.identifier.issn0002-7863
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/276383
dc.description.abstractBased on the saposin-A (SapA) scaffold protein we demonstrate the suitability of a size-adaptable phospholipid membrane-mimetic system for solution NMR studies of membrane proteins under close-to-native conditions. The Salipro nanoparticle size can be tuned over a wide pH range by adjusting the saposin-to-lipid stoichiometry enabling maintenance of sufficiently high amounts of phospholipid in the Salipro nanoparticle to mimic a realistic membrane environment while controlling the overall size to enable solution NMR for a range of membrane proteins. Three representative membrane proteins including one G-protein-coupled receptor (GPCR) were successfully incorporated into SapA-dimyristoylphosphatidylcholine (DMPC) nanoparticles and studied by solution NMR spectroscopy.
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectSaposinsen
dc.subjectPhospholipidsen
dc.subjectDimyristoylphosphatidylcholineen
dc.subjectMembrane Proteinsen
dc.subjectReceptors, G-Protein-Coupleden
dc.subjectMembranes, Artificialen
dc.subjectMagnetic Resonance Spectroscopyen
dc.subjectBiomimeticsen
dc.subjectHydrogen-Ion Concentrationen
dc.subjectNanoparticlesen
dc.titleAn Adaptable Phospholipid Membrane Mimetic System for Solution NMR Studies of Membrane Proteins.en
dc.typeArticle
prism.endingPage14832
prism.issueIdentifier42en
prism.publicationDate2017en
prism.publicationNameJournal of the American Chemical Societyen
prism.startingPage14829
prism.volume139en
dc.identifier.doi10.17863/CAM.23687
dcterms.dateAccepted2017-10-09en
rioxxterms.versionofrecord10.1021/jacs.7b06730en
rioxxterms.versionVoR*
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2017-10-10en
dc.contributor.orcidChien, Chih-Ta Henry [0000-0002-7796-0937]
dc.contributor.orcidBostock, Mark [0000-0001-6717-5786]
dc.contributor.orcidTan, Yi Lei [0000-0002-4013-3225]
dc.contributor.orcidNietlispach, Daniel [0000-0003-4364-9291]
dc.identifier.eissn1520-5126
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/K01983X/1)
pubs.funder-project-idMRC (MR/L014254/1)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International