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dc.contributor.authorToshner, Mark
dc.contributor.authorDunmore, Benjamin
dc.contributor.authorMcKinney, Eoin
dc.contributor.authorSouthwood, Mark
dc.contributor.authorCaruso, Paola
dc.contributor.authorUpton, Paul
dc.contributor.authorWaters, John
dc.contributor.authorOrmiston, Mark
dc.contributor.authorSkepper, Jeremy N
dc.contributor.authorNash, Gerard
dc.contributor.authorRana, Moo
dc.contributor.authorMorrell, Nicholas
dc.date.accessioned2018-06-14T14:48:21Z
dc.date.available2018-06-14T14:48:21Z
dc.date.issued2014
dc.identifier.issn1932-6203
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/277052
dc.description.abstractThe endothelial cell has a remarkable ability for sub-specialisation, adapted to the needs of a variety of vascular beds. The role of developmental programming versus the tissue contextual environment for this specialization is not well understood. Here we describe a hierarchy of expression of HOX genes associated with endothelial cell origin and location. In initial microarray studies, differential gene expression was examined in two endothelial cell lines: blood derived outgrowth endothelial cells (BOECs) and pulmonary artery endothelial cells. This suggested shared and differential patterns of HOX gene expression between the two endothelial lines. For example, this included a cluster on chromosome 2 of HOXD1, HOXD3, HOXD4, HOXD8 and HOXD9 that was expressed at a higher level in BOECs. Quantative PCR confirmed the higher expression of these HOXs in BOECs, a pattern that was shared by a variety of microvascular endothelial cell lines. Subsequently, we analysed publically available microarrays from a variety of adult cell and tissue types using the whole "HOX transcriptome" of all 39 HOX genes. Using hierarchical clustering analysis the HOX transcriptome was able to discriminate endothelial cells from 61 diverse human cell lines of various origins. In a separate publically available microarray dataset of 53 human endothelial cell lines, the HOX transcriptome additionally organized endothelial cells related to their organ or tissue of origin. Human tissue staining for HOXD8 and HOXD9 confirmed endothelial expression and also supported increased microvascular expression of these HOXs. Together these observations suggest a significant involvement of HOX genes in endothelial cell positional identity.
dc.format.mediumElectronic-eCollection
dc.languageeng
dc.publisherPublic Library of Science (PLoS)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectLung
dc.subjectPulmonary Artery
dc.subjectCell Line
dc.subjectEndothelial Cells
dc.subjectFetus
dc.subjectAnimals
dc.subjectHumans
dc.subjectMice
dc.subjectRats
dc.subjectHomeodomain Proteins
dc.subjectRNA, Messenger
dc.subjectOligonucleotide Array Sequence Analysis
dc.subjectCluster Analysis
dc.subjectReproducibility of Results
dc.subjectGene Expression Profiling
dc.subjectCell Differentiation
dc.subjectOrgan Specificity
dc.subjectNeovascularization, Physiologic
dc.subjectPhenotype
dc.subjectGenes, Homeobox
dc.subjectAdult
dc.subjectEmbryonic Stem Cells
dc.subjectReal-Time Polymerase Chain Reaction
dc.subjectGene Ontology
dc.titleTranscript analysis reveals a specific HOX signature associated with positional identity of human endothelial cells.
dc.typeArticle
prism.issueIdentifier3
prism.publicationDate2014
prism.publicationNamePLoS One
prism.startingPagee91334
prism.volume9
dc.identifier.doi10.17863/CAM.24352
dcterms.dateAccepted2014-02-10
rioxxterms.versionofrecord10.1371/journal.pone.0091334
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2014-01
dc.contributor.orcidToshner, Mark [0000-0002-3969-6143]
dc.contributor.orcidMcKinney, Eoin [0000-0003-3516-3072]
dc.contributor.orcidUpton, Paul [0000-0003-2716-4921]
dc.contributor.orcidRana, Moo [0000-0002-2330-4643]
dc.contributor.orcidMorrell, Nicholas [0000-0001-5700-9792]
dc.identifier.eissn1932-6203
rioxxterms.typeJournal Article/Review
pubs.funder-project-idMedical Research Council (G1000847)
pubs.funder-project-idBritish Heart Foundation (None)
pubs.funder-project-idBritish Heart Foundation (None)
pubs.funder-project-idBritish Heart Foundation (None)
pubs.funder-project-idMedical Research Council (MR/K020919/1)
pubs.funder-project-idBritish Heart Foundation (None)
pubs.funder-project-idBritish Heart Foundation (None)
pubs.funder-project-idBritish Heart Foundation (None)
pubs.funder-project-idMedical Research Council (G0800784)
cam.issuedOnline2014-03-20


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International